Recombinant Mouse Netrin-1 Protein

Name: Recombinant Mouse Netrin-1 Protein
Brand: R&D Systems
SKU: 1109-N1
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1109-N1

Citations (33)

(3)

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Key Product Details

Source:	NS0
Accession #:	AAC52971
Applications:	Binding Activity

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Product Specifications for Recombinant Mouse Netrin-1 Protein

Source

Mouse myeloma cell line, NS0-derived mouse Netrin-1 protein

Mouse Netrin-1 (Val22-Ala603) Accession # AAC52971	IEGR	GGGSGGGSGGGS	10-His tag
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Val22

Predicted Molecular Mass

68.2 kDa

SDS-PAGE

83 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant rat UNC5H2 Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Mouse Netrin-1 with a linear range of 3‑200 ng/mL.

Scientific Data Examples for Recombinant Mouse Netrin-1 Protein

	Recombinant Mouse Netrin-1 Protein SDS-PAGE 1 μg/lane of Recombinant Mouse Netrin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 83 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1109-N1

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1109-N1/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Netrin-1

Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma ‑chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin-1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis, Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1‑7).

Item	Value
References	Puschel, A. (1999) Mech. Dev. 83:65. Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287. Madison, R. et al. (2000) Exp. Neurology 161:563. Srinivasan, K. et al. (2003) Dev. Cell 4:371. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62. Corset, V. et al. (2000) Nature 407:747.
Entrez Gene IDs	9423 (Human); 18208 (Mouse); 396389 (Chicken)
Alternate Names	NTN1, NTN1Lnetrin 1, mouse, homolog of, Netrin-1, Netrin1, netrin 1

Product Specific Notices for Recombinant Mouse Netrin-1 Protein

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

Citations for Recombinant Mouse Netrin-1 Protein (33)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Netrin-1 Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Boyé et al. (2022), Endothelial Unc5B controls blood-brain barrier integrity Nature Communications, 13(1):1169.
PMID: 35246514
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

V Barroca et al. (2022), The netrin-1 receptor UNC5C contributes to the homeostasis of undifferentiated spermatogonia in adult mice Stem Cell Research, 60(0):102723.
PMID: 35247845
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

H Wei et al. (2022), Spinal TRPA1 Contributes to the Mechanical Hypersensitivity Effect Induced by Netrin-1 International Journal of Molecular Sciences, 23(12).
PMID: 35743067
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

G Chen et al. (2021), Netrin-1 receptor UNC5C cleavage by active &delta-secretase enhances neurodegeneration, promoting Alzheimer's disease pathologies Science Advances, 7(16).
PMID: 33863723
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

D Pradella et al. (2021), A ligand-insensitive UNC5B splicing isoform regulates angiogenesis by promoting apoptosis Nature Communications, 12(1):4872.
PMID: 34381052
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Zhuang et al. (2019), The m6A reader YTHDF1 regulates axon guidance through translational control of Robo3.1 expression Nucleic Acids Res., 0(0).
PMID: 30843071
Species: Chicken
Sample Types: Whole Tissue
Applications: Bioassay

A Pignata et al. (2019), A Spatiotemporal Sequence of Sensitization to Slits and Semaphorins Orchestrates Commissural Axon Navigation Cell Rep, 29(2):347-362.e5.
PMID: 31597096
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

T Shigeoka et al. (2019), On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons Cell Rep, 29(11):3605-3619.e10.
PMID: 31825839
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Q Shao et al. (2019), Disease-associated mutations in human TUBB3 disturb netrin repulsive signaling PLoS ONE, 14(6):e0218811.
PMID: 31226147
Species: Mouse
Sample Types: In Vivo

Y Zhang et al. (2018), Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor Sci Rep, 8(1):5994.
PMID: 29662125
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

X He et al. (2018), Netrin-1 attenuates brain injury after middle cerebral artery occlusion via downregulation of astrocyte activation in mice J Neuroinflammation, 15(1):268.
PMID: 30227858
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

T Sato et al. (2017), Functional Roles of Netrin-1 in Osteoblast Differentiation In Vivo, 31(3):321-328.
PMID: 28438858
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

KA Glendining et al. (2017), A novel role for the DNA repair gene Rad51 in Netrin-1 signalling Sci Rep, 7(0):39823.
PMID: 28057929
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

K Yamahara et al. (2017), Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells Neuropharmacology, 119(0):26-39.
PMID: 28373074
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Hashimoto et al. (2016), An Alzheimer's disease-linked rare mutation potentiates netrin receptor uncoordinated-5C-induced signaling that merges with amyloid ? precursor protein signaling J Biol Chem, 0(0).
PMID: 27068745
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

Ros O et al. (2015), Regulation of patterned dynamics of local exocytosis in growth cones by netrin-1. J Neurosci, 35(13):5156-70.
PMID: 25834042
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

O'Leary C et al. (2015), The Netrin/RGM receptor, Neogenin, controls adult neurogenesis by promoting neuroblast migration and cell cycle exit. Stem Cells, 33(2):503-14.
PMID: 25308084
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Mediero A et al. (2015), Netrin-1 is a critical autocrine/paracrine factor for osteoclast differentiation. J Bone Miner Res, 30(5):837-54.
PMID: 25483983
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lu et al. (2014), The Parkinsonian mimetic, 6-OHDA, impairs axonal transport in dopaminergic axons. Mol Neurodegener, 9(0):17.
PMID: 24885281
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ramkhelawon B et al. (2014), Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med, 20(4):377-84.
PMID: 24584118
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Chen J et al. (2012), Netrin-1 protects against L-Arginine-induced acute pancreatitis in mice. PLoS ONE, 7(9):e46201.
PMID: 23029434
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Palmesino E et al. (2012), Genetic analysis of DSCAM's role as a Netrin-1 receptor in vertebrates. J. Neurosci., 32(2):411-6.
PMID: 22238077
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Kim-Han JS et al. (2011), The Parkinsonian mimetic, MPP+, specifically impairs mitochondrial transport in dopamine axons. J. Neurosci., 31(19):7212-21.
PMID: 21562285
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Kuwako K et al. (2010), Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression. Neuron, 67(3):407-21.
PMID: 20696379
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Tadagavadi RK et al. (2010), Netrin-1 Regulates Th1/Th2/Th17 Cytokine Production and Inflammation through UNC5B Receptor and Protects Kidney against Ischemia-Reperfusion Injury. J. Immunol., 185(0):3750-8.
PMID: 20693423
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Rosenberger P et al. (2009), Hypoxia-inducible factor-dependent induction of netrin-1 dampens inflammation caused by hypoxia. Nat. Immunol., 10(2):195-202.
PMID: 19122655
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Mancino M et al. (2009), Neuronal guidance protein Netrin-1 induces differentiation in human embryonal carcinoma cells. Cancer Res., 69(5):1717-21.
PMID: 19223540
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Schaffar G et al. (2008), LIM-only protein 4 interacts directly with the repulsive guidance molecule A receptor Neogenin. J. Neurochem., 107(2):418-31.
PMID: 18702663
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Strizzi L et al. (2008), Netrin-1 can affect morphogenesis and differentiation of the mouse mammary gland. J. Cell. Physiol., 216(3):824-34.
PMID: 18425773
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Wu TW et al. (2008), Netrin-1 attenuates ischemic stroke-induced apoptosis. Neuroscience, 156(3):475-82.
PMID: 18786616
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Wang W et al. (2008), Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney. Am. J. Physiol. Renal Physiol., 294(4):F739-47.
PMID: 18216145
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay

Isakova IA et al. (2007), Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain. Stem Cells, 25(12):3261-70.
PMID: 17932418
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay

Nguyen A et al. (2006), Netrin-1 induces angiogenesis via a DCC-dependent ERK1/2-eNOS feed-forward mechanism. Proc. Natl. Acad. Sci. U.S.A., 103(17):6530-5.
PMID: 16611730

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Recombinant Mouse Netrin-1 Protein

Name: Lenin Veeraval

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 06/18/2022
Name: Lenin Veeraval

Application: Stem/Immune cell maintenance or differentiation

Reason for Rating: Could see a concentration dependent increase in the protein expression in the western blot.

Verified Customer | Posted 06/14/2022

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Recombinant Mouse Netrin-1 Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 05/12/2016

Recombinant Mouse Netrin-1 Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Mouse Netrin-1 Protein

Scientific Data Examples for Recombinant Mouse Netrin-1 Protein

Formulation, Preparation and Storage

Carrier Free

1109-N1

Carrier

1109-N1/CF

Carrier Free

Reconstitution Calculator

Background: Netrin-1

Product Specific Notices for Recombinant Mouse Netrin-1 Protein

Citations for Recombinant Mouse Netrin-1 Protein (33)

Customer Reviews for Recombinant Mouse Netrin-1 Protein (3)

Customer Images

Recombinant Mouse Netrin-1 Protein

Recombinant Mouse Netrin-1 Protein

Product Documents for Recombinant Mouse Netrin-1 Protein

Product Datasheets

COA

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