Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF

Name: Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10111-JG

Catalog # 10111-JG | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	P78504
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF

Technical Data
Citations (1) / Reviews

Product Specifications for Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human Jagged 1 protein

Human Jagged 1 (Ser32-Ile335) Accession # P78504	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser32

Predicted Molecular Mass

61 kDa

SDS-PAGE

61 kDa

Activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED₅₀ for this effect is 0.3-1.8 μg/mL.

Scientific Data Examples for Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF

	Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein Bioactivity Immobilized Recombinant Human Jagged 1 N-Terminal Fc Chimera (Catalog # 10111-JG) enhances Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.3-1.8 µg/mL.
	Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human Jagged 1 N-Terminal Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 72-80 kDa and 140-160 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10111-JG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. This product is an N-terminal ECD fragment that includes DSL domain and the first 3 EGF-like domains. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

Item	Value
References	Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849. Artavamis-Tsokanas, S. et al. (1999) Science 284:770. Lai, E.C. (2004) Development 131:965. Milner, L.A. et al. (1994) Blood 83:2057. Nyfeler, Y. et al. (2005) EMBO J. 24:3504. Linheng, L. et al. (1998) Immunity 8:43. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543. Oda, T. et al. (1997) Nat. Genet. 16:235. Oda, T. et al. (1997) Genomics 43:376.
Entrez Gene IDs	182 (Human); 16449 (Mouse); 29146 (Rat)
Alternate Names	AGS, AHDMGC104644, AWS, Alagille syndrome, CD339, CD339 antigen, HJ1, JAG1, JAGL1, Jagged 1, Jagged1, protein jagged-1

Citations for Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JI Choi et al. (2022-05-24), The role of Jagged1 as a dynamic switch of cancer cell plasticity in PDAC assembloids Theranostics, 2022-012(9):4431-4445.
PMID: 35673567

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Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF

Catalog # 10111-JG | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Jagged 1

Citations for Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF (1)

Product Documents for Recombinant Human Jagged 1 N-Terminal Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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