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Recombinant Human IL-6R alpha His-tag Protein, CF

Catalog # 10537-SR | R&D Systems, Inc. a Bio-Techne Brand
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10537-SR-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-6R alpha protein
Leu20-Pro365, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

39 kDa

SDS-PAGE

67-73 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human IL-6 (Catalog # 7270-IL) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human IL-6R alpha His-tag (Catalog # 10537-SR) binds with an ED50 of 0.400-3.60 μg/mL.

Scientific Data Images for Recombinant Human IL-6R alpha His-tag Protein, CF

Recombinant Human IL-6R alpha His-tag Protein Binding Activity.

When Recombinant Human IL-6 (7270-IL) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human IL-6R alpha His-tag Protein (Catalog # 10537-SR) binds with an ED50 of 0.400-3.60 μg/mL.

Recombinant Human IL-6R alpha His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL-6R alpha His-tag Protein (Catalog # 10537-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-73 kDa.

Formulation, Preparation and Storage

10537-SR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6R alpha

Interleukin-6 receptor subunit alpha (IL-6Ra), also known as membrane glycoprotein 80 (gp80), IL-6R 1 or CD126, is a single-pass type I membrane protein of the interleukin receptor family. Human IL-6Ra is an 80 kDa receptor component that binds IL-6 with low affinity and forms one-half of the IL-6R receptor complex. IL-6Ra associates with a non-ligand binding 130 kDa glycoprotein signal-transducing component (CD130; gp130) for high-affinity binding to IL-6 (1-4). When bound to IL-6, the IL-6R complex forms a trimer, which homodimerizes to form a hexameric cellular signaling complex (1, 4). Human IL-6Ra consists of an extracellular domain (ECD) with an N-terminal Ig-like C2-type domain and a cytokine-binding domain containing two fibronectin type-III domains with a WSXWS motif, a helical transmembrane segment and a cytoplasmic domain. Within the mature ECD, human IL-6Ra shares 51% and 52% amino acid sequence identity with mouse and rat IL-6Ra, respectively. Soluble forms of IL-6Ra can be generated via ADAM10 and ADAM17 cleavage of membrane bound IL-6Ra, alternative mRNA splicing and microvesicle release (3, 4). Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn's disease (6, 7). 

References

  1. Hansen, Morton. (2020) Immun. Inflamm. Dis. 8:170.
  2. Salem, D. et al. (2018) Leuk Lymphoma. 59:178.
  3. Dayer, J. and Choy, E. (2009) Rheumatology. 49:15.
  4. Schumacher, N. et al. (2015) J. Biol. Chem. 290:26059.
  5. Novick, D. et al. (1989) J. Exp. Med. 170:1409.
  6. Jones, S.A. et al. (2001) FASEB J. 15:43.
  7. Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.

Long Name

Interleukin 6 Receptor alpha

Alternate Names

CD126, IL-6 R alpha, IL-6Ra, IL6R, IL6R alpha

Entrez Gene IDs

3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)

Gene Symbol

IL6R

UniProt

Product Documents for Recombinant Human IL-6R alpha His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-6R alpha His-tag Protein, CF

For research use only

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