The specifications section on the website for Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, describes SDS-PAGE as a range of Molecular Weights. Why?

Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, has one N-linked glycosylation site and seven O-linked glycosylation sites. The variable glycosylation results in multiple bands for this protein.

What are the diferences between Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB) and Recombinant Human IL-17A (Human Cell-expressed) Protein (Catalog # 7955-IL)?

There are multiple differences between Catalog # 317-ILB and 7955-IL. 317-ILB is raised in E. coli, while 7955-IL is raised in Human Embryonic Kidney (HEK293) cells. Human Cell-expressed 7955-IL has a lower endotoxin specification (<0.01 EU per 1 μg of the protein by the LAL method) compared to E. coli-expressed 317-ILB (<0.10 EU per 1 μg of the protein). Human Cell-expressed 7955-IL exhibits greater activity in the CXCL1/GRO alpha secretion assay in HT‑29 human colon adenocarcinoma cells (0.12 - 1.2 ng/mL) compared to E. coli-expressed 317-ILB (0.4-4.0 ng/mL).

Recombinant Human IL-17A (Human Cell-expressed) Protein

Name: Recombinant Human IL-17A (Human Cell-expressed) Protein
Brand: R&D Systems
SKU: 7955-IL
Rating: 4 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7955-IL

Citations (14)

(3)

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Key Product Details

Source:	HEK293
Accession #:	Q16552
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human IL-17A (Human Cell-expressed) Protein

Source	Human embryonic kidney cell, HEK293-derived human IL-17/IL-17A protein Gly24-Ala155
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gly24
Predicted Molecular Mass	15.1 kDa (monomer)
SDS-PAGE	15-23 kDa, reducing conditions 28-38 kDa, non-reducing conditions
Activity	Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.12-1.2 ng/mL. Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED₅₀ for this effect is 1.5-7.5 ng/mL.

Scientific Data Examples for Recombinant Human IL-17A (Human Cell-expressed) Protein

	Recombinant Human IL-17A (Human Cell-expressed) Protein Bioactivity Recombinant Human IL-17 (Catalog # 7955-IL) induces CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.12‑1.2 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

7955-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7955-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17/IL-17A

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL‑17A shares 60% amino acid sequence identity with mouse and rat IL‑17A (3, 4). IL‑17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide‑linked homodimers as well as disulfide‑linked heterodimers with IL‑17F (5, 6). IL‑17A exerts its effects through the transmembrane IL‑17 RA in complex with IL‑17 RC or IL‑17 RD (7, 8). Both IL‑17 RA and IL‑17 RC are required for responsiveness to heterodimeric IL‑17A/F (7). IL‑17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL‑17A/F likewise induces neutrophil migration, but IL‑17F does not (11). IL‑17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF‑ alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL‑17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15). IL‑17A has been shown to exert either tumorigenic or anti‑tumor effects (16, 17).

Item	Value
References	Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479. Yao, Z. et al. (1995) J. Immunol. 155:5483. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593. Chang, S.H. and C. Dong (2007) Cell Res. 17:435. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447. Wright, J.F. et al. (2008) J. Immunol. 181:2799. Rong, Z. et al. (2009) Cell Res. 19:208. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271. Liang, S.C. et al. (2007) J. Immunol. 179:7791. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755. Hsu, H. et al. (2008) Nat. Immunol. 9:166. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857. Wang, L. et al. (2009) J. Exp. Med. 206:1457. Kryczek, I. et al. (2009) Blood 114:357.
Long Name	Interleukin 17
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names	CTLA-8, CTLA8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8, IL17, IL17A, IL17interleukin-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A

Citations for Recombinant Human IL-17A (Human Cell-expressed) Protein (14)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-17A (Human Cell-expressed) Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

G Margetts et al. (2022), Evidence for anti-inflammatory effects and modulation of neurotransmitter metabolism by Salvia officinalis L BMC complementary medicine and therapies, 22(1):131.
PMID: 35550086
Species: Human
Sample Types: Organoid
Applications: Bioassay

P Pavlidis et al. (2022), Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease Cell Reports, 40(13):111439.
PMID: 36170836
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

EM Byrne et al. (2021), IL-17A Damages the Blood-Retinal Barrier through Activating the Janus Kinase 1 Pathway Biomedicines, 9(7).
PMID: 34356895
Species: Bovine
Sample Types: Whole Tissue
Applications: Bioassay

D Sinkeviciu et al. (2020), Characterization of the interleukin-17 effect on articular cartilage in a translational model: an explorative study BMC Rheumatol, 4(0):30.
PMID: 32426694
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Sun et al. (2020), IL-10 Dampens an IL-17-Mediated Periodontitis-Associated Inflammatory Network J. Immunol., 204(8):2177-2191.
PMID: 32169848
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JE Heath et al. (2019), IL-36? regulates mediators of tissue homeostasis in epithelial cells Cytokine, 119(0):24-31.
PMID: 30856602
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

BK Tusi et al. (2018), Population snapshots predict early haematopoietic and erythroid hierarchies Nature, 0(0).
PMID: 29466336
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

ED Anderson et al. (2018), Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632 PLoS ONE, 13(9):e0198862.
PMID: 30208113
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay

J Chorachoo et al. (2018), The small molecule rhodomyrtone suppresses TNF-? and IL-17A-induced keratinocyte inflammatory responses: A potential new therapeutic for psoriasis PLoS ONE, 13(10):e0205340.
PMID: 30321197
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Bambouskov et al. (2018), Electrophilic properties of itaconate and derivatives regulate theï¿½I?B?-ATF3 inflammatory axis Nature, 556(7702):501-504.
PMID: 29670287
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Muntaka et al. (2018), IFN-? and IL-17A differentially influence the response of human macrophages and neutrophils to Pseudomonas aeruginosa infection Infect. Immun., 0(0).
PMID: 30455194
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

WR Swindell et al. (2018), RNA-Seq Analysis of IL-1B and IL-36 Responses in Epidermal Keratinocytes Identifies a Shared MyD88-Dependent Gene Signature Front Immunol, 9(0):80.
PMID: 29434599
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

BA Stanfield et al. (2018), Intramuscular vaccination of guinea pigs with the live-attenuated human herpes simplex vaccine VC2 stimulates a transcriptional profile of vaginal Th17 and regulatory Tr1 responses Vaccine, 0(0).
PMID: 29655629
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Impaired Tight Junctions in Atopic Dermatitis Skin and in a Skin-Equivalent Model Treated with Interleukin-17 PLoS ONE, 11(9):e0161759.
PMID: 27588419

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Customer Reviews for Recombinant Human IL-17A (Human Cell-expressed) Protein (3)

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Application: In vitro bioactivity in cell culture

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FAQs for Recombinant Human IL-17A (Human Cell-expressed) Protein

The specifications section on the website for Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, describes SDS-PAGE as a range of Molecular Weights. Why?

Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, has one N-linked glycosylation site and seven O-linked glycosylation sites. The variable glycosylation results in multiple bands for this protein.
What are the diferences between Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB) and Recombinant Human IL-17A (Human Cell-expressed) Protein (Catalog # 7955-IL)?
There are multiple differences between Catalog # 317-ILB and 7955-IL.
- 317-ILB is raised in E. coli, while 7955-IL is raised in Human Embryonic Kidney (HEK293) cells.
- Human Cell-expressed 7955-IL has a lower endotoxin specification (<0.01 EU per 1 μg of the protein by the LAL method) compared to E. coli-expressed 317-ILB (<0.10 EU per 1 μg of the protein).
- Human Cell-expressed 7955-IL exhibits greater activity in the CXCL1/GRO alpha secretion assay in HT‑29 human colon adenocarcinoma cells (0.12 - 1.2 ng/mL) compared to E. coli-expressed 317-ILB (0.4-4.0 ng/mL).

Recombinant Human IL-17A (Human Cell-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human IL-17A (Human Cell-expressed) Protein

Scientific Data Examples for Recombinant Human IL-17A (Human Cell-expressed) Protein

Formulation, Preparation and Storage

Carrier Free

7955-IL

Carrier

7955-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-17/IL-17A

Citations for Recombinant Human IL-17A (Human Cell-expressed) Protein (14)

Customer Reviews for Recombinant Human IL-17A (Human Cell-expressed) Protein (3)

Recombinant Human IL-17A (Human Cell-expressed) Protein

Recombinant Human IL-17A (Human Cell-expressed) Protein

FAQs for Recombinant Human IL-17A (Human Cell-expressed) Protein

Product Documents for Recombinant Human IL-17A (Human Cell-expressed) Protein

Product Datasheets

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