Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein, CF

Name: Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI10930

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AVI10930

Product Datasheet / COA / SDS

Biotinylated

Catalog #

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Qty

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	Q99665.1
Structure / Form:	Disulfide-linked homodimer Biotinylated via Avi-tag
Applications:	Bioactivity

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Product Specifications for Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-12 R beta 2 protein

Human IL-12 RB2 (Cys28-Asn622) Accession # Q99665.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Cys28

Predicted Molecular Mass

96 kDa

SDS-PAGE

115-130 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-12 Protein (Catalog # 219-IL) is immobilized at 1.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL‑12 R beta 2 Fc Chimera Avi-tag (Catalog# AVI10930) that produces 50% of the optimal binding response is 0.15‑1.50 μg/mL.

Scientific Data Examples for Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein, CF

	When Recombinant Human IL-12 Protein (Catalog# 219-IL) is immobilized at 1.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL‑12R beta 2 Fc Chimera Avi-tag (Catalog # AVI10930) that produces 50% of the optimal binding response is 0.15‑1.50 μg/mL.
	Biotinylated Recombinant Human IL‑12 R beta 2 Fc Chimera Avi-tag Protein SDS-PAGE. 1 μg/lane of Biotinylated Recombinant Human IL‑12 R beta 2 Fc Chimera Avi-tag Protein (Catalog # AVI10930) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized bysilver staining, showing bands at 115-130 kDa and 230-260 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10930

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-12 R beta 2

IL-12 Receptor beta 2 (IL-12 R beta 2) is a member of the cytokine receptor superfamily and when combined with the highly homologous IL-12 R beta 1, forms the heterodimeric high affinity IL-12 receptor complex. Both receptors are type I transmembrane glycoproteins with homology to gp130, the common receptor beta -chain of the IL-6-like cytokine superfamily (ref). Mature human IL-12 R beta 2 consists of an extracellular domain (ECD) with five fibronectin type III (FnIII) domains and a WSXWS motif, a single transmembrane domain and a cytoplasmic region with a Box 1 motif and a tyrosine phosphorylation site that both mediate intracellular signaling (1). The ECD of human IL-12 R beta 2 shares 69% and 67% amino acid sequence identity with mouse and rat IL-12 R beta 2, respectively. Human and mouse IL-12 R beta 2 do not bind cross-species IL-12 (2). Alternative splicing creates a IL-12 R beta 2 with a shortened and altered cytoplasmic sequence (3). IL-12 R beta 1 and IL-12 R beta 2 are receptors for the IL-12 family, which includes IL-12, IL-23, IL-27 and IL-35 and they activate the Janus kinase (JAK)–STAT (signal transducer and activator of transcription) pathway of signal transduction (4). The receptor chains are utilized by the cytokines in different combinations, with IL-12RB2 binding to IL-12 and IL-35 (5). Unlike IL-12RB1, which is constitutive in T cells, NK cells and B cells, IL-12 R beta 2 expression is more limited (2). IL-12 R beta 2 is expressed following STAT1 activation by IFN-gamma, IL-27 and/or T cell receptor stimulation of naïve T cells, allowing IL-12 to promote Th1, but not Th2, differentiation (6-8). Among B cells, surface expression is limited to naïve germinal center and memory B cells, and myeloma cells (2). Deletion of mouse IL-12 R beta 2 causes systemic overexpression of IL-6, accelerated maturation of thymocytes, deficient regulatory T cell maturation and function, and reduced splenic T cell apoptosis (2, 9-11). These mice are susceptible to autoimmune diseases such as experimental autoimmune encephalitis and spontaneous B cell malignancies (2, 9-11). In humans, polymorphism of the IL-12 R beta 2 gene is associated with systemic sclerosis (12). Our Avi-tag Biotinylated IL-12 R beta 2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Item	Value
References	Zou, J. et al. (1997) J. Biol. Chem. 272:6073. Pistoia, V. et al. (2009) J. Clin. Oncol. 27:4809. Poltorak, A. et al. (2001) J Immunol 167:2106. Vignali, D.A. and Kuchroo, V.K. (2012) Immunol. 13:722. Jones, L.L. and Vignali, D.A. (2011) Immunol Res. 51:5. Rogge, L. et al. (1997) J. Exp. Med. 185:825. Becskei, A. and M.J. Grusby (2007) FEBS Lett. 581:5199. Szabo, S.J. et al. (1997) J. Exp. Med. 185:817. Zhao, Z. et al. (2008) J. Immunol. 181:3870. Gran, B. et al. (2010) Exp. Mol. Pathol. 89:126. Airoldi, I. et al. (2005) Blood 106:3846. Bossini-Castillo, L. et al. (2012) Hum. Mol. Genet. 21:926.
Long Name	Interleukin 12 Receptor beta 2
Entrez Gene IDs	3595 (Human); 16162 (Mouse)
Alternate Names	IL-12 R beta 2, IL-12 receptor beta 2, IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, IL-12Rb2, IL12R beta 2, IL12RB2, interleukin 12 receptor, beta 2, interleukin-12 receptor beta-2 chain, interleukin-12 receptor subunit beta-2

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