Recombinant Human ICAM-4 Fc Chimera Protein, CF

Name: Recombinant Human ICAM-4 Fc Chimera Protein, CF
Brand: rnd
SKU: 10407-IC

Catalog # 10407-IC

Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	Q14773.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human ICAM-4 Fc Chimera Protein, CF

Technical Data
Citations / Reviews / FAQs

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ICAM-4 protein

Human ICAM-4 (Ala31-Ala240) Accession # Q14773.1	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala31

Predicted Molecular Mass

50 kDa

SDS-PAGE

50-60 kDa & 70-80 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Integrin alpha L beta 2 (Catalog # 3868-AV) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human ICAM-4 Fc Chimera (Catalog # 10407-IC) binds with an ED₅₀ of 0.25-1.5 μg/mL.

Recombinant Human ICAM-4 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Human ICAM-4 Fc Chimera Protein Binding Activity When Recombinant Human Integrin alpha L beta 2 Protein (3868-AV) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human ICAM-4 Fc Chimera (10407-IC) binds with an ED₅₀ of 0.25-1.5 μg/mL.
	Recombinant Human ICAM-4 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human ICAM-4 Fc Chimera (Catalog # 10407-IC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-60 kDa & 70-80 kDa, and 140-160 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10407-IC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICAM-4

ICAM-4 (intercellular adhesion molecule-4), also known as CD242, is a transmembrane cell adhesion glycoprotein and a member of the immunoglobulin protein superfamily. The ICAM sub-family consists of five members, ICAM-1 through ICAM-5, and they vary in their tissue expression and number of Ig-like domains in the extracellular domain (ECD) (1). Full-length human ICAM-4 contains 2 Ig-like domains in the ECD, a single transmembrane domain and short intracellular domain. Alternative splicing of ICAM-4 results in at least one soluble form (2). The ECD of mature human ICAM-4 shares 68% and 67% amino acid sequence identity with mouse and rat ICAM-4, respectively. ICAM-4 expression is limited to erythroid and possibly placental tissue but its biological role remains poorly defined (3). ICAM-4 has been shown to bind alpha 4 beta 1 and alpha V family integrins as well as displaying broad ligand binding specificity for some beta 1, beta 2, beta 3 and beta 5 integrins (4, 5). ICAM-4 binding to endothelial alpha v beta 3 has been indicated as a factor in vaso-occlusion, particularly in sickle cell disease (6). ICAM-4 is expressed on red blood cells (RBC), erythroid precursor cells, and possibly placental tissue (3, 7). ICAM-4 has shown to bind alpha 4 beta 1 on hemopoietic cells, alpha V family integrins (avb1, avb3, and avb5) on non-hemopoietic cells as well as displaying broad ligand binding specificity for some beta 1, beta 2, beta 3 and beta 5 integrins (4, 5, 7). Studies have shown aLb2 integrin interacts through the first Ig-like domain of ICAM-4, whereas aMb2 and aXb2 integrins interact through both Ig-like domains of ICAM-4 (7). In addition, initiation of vaso-occlusion in sickle cell disease is implicated by ICAM-4 binding to endothelial alpha v beta 3 integrin (6). The ability of ICAM-4 to interact selectively with different integrins suggests its importance in RBC physiology and pathology as well as its therapeutic value.

Item	Value
References	Gahmberg, C.G. et al. (1997) Eur. J. Biochem. 245, 215. Lee, G. et al. (2003) Blood 101:1790. Southcott, M.J.G. et al. (1999) Blood 93:4425. Spring, F.A. et al. (2001) Blood. 98:458. Hermand, P. et al. (2003) J Biol Chem. 278:4892. Kaul, D.K. et al. (2006) Am J Physiol Cell Physiol. 291:C922. Ihanus, E. et al. (2007) Blood 109:802.
Long Name	Intercellular Adhesion Molecule 4
Entrez Gene IDs	3386 (Human); 78369 (Mouse)
Alternate Names	CD242, CD242 antigen, ICAM-4, ICAM4, LWLW blood group protein, Landsteiner-Wiener blood group, Landsteiner-Wiener blood group antigen a, Landsteiner-Wiener blood group glycoprotein, intercellular adhesion molecule 4, intercellular adhesion molecule 4 (LW blood group), intercellular adhesion molecule 4 (Landsteiner-Wiener blood group), intercellular adhesion molecule 4, Landsteiner-Wiener blood group

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