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Recombinant Human Integrin alpha L beta 2 Protein, CF
Catalog # 3868-AV | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Source
CHO
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha L beta 2 protein
| Human Integrin alphaL (Tyr26-Met1089) Accession # P20701 |
acidic tail |
| Human Integrin beta2 (Gln23-Asn700) Accession # P05107 |
basic tail |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Tyr26 (Integrin alphaL) & No results obtained: Gln23 predicted (Integrin beta2)
Predicted Molecular Mass
121.7 kDa (Integrin alphaL), 79 kDa (Integrin beta2)
SDS-PAGE
160 kDa and 100 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with ICAM-1.
When 5 x 104 cells/well are added to Integrin alphaL beta2-coated plates (5 µg/mL, 100 µL/well), 60‑80% of the cells will adhere after 1 hour at 37° C in the presence of 1 mM MnCl2.
When 5 x 104 cells/well are added to Integrin alphaL beta2-coated plates (5 µg/mL, 100 µL/well), 60‑80% of the cells will adhere after 1 hour at 37° C in the presence of 1 mM MnCl2.
Formulation, Preparation and Storage
3868-AV
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Integrin alpha L beta 2
Integrin alphaL beta2, also called LFA-1, is one of three beta2 integrin adhesion proteins. The non-covalent heterodimer of 180 kDa alphaL/CD11a and 95 kDa beta2/CD18 integrin subunits is expressed on virtually all leukocytes (1-3). The ligand binding site of LFA-1 is in the N-terminal head region, formed by an interaction of the vWFA (I, I‑like) domains from each subunit, and the alphaL beta-propeller structure (3-5). The alphaL subunit contains domains termed thigh, calf-1 and calf-2, while the beta2 subunit contains a PSI (plexin-semaphorin-integrin) region and four cysteine-rich I-EGF folds (5, 6). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Upon activation by “inside-out” signaling, clustering, or Mg2+ or Mn2+ binding to metal ion-dependent adhesion sites (MIDAS) within the vWFA domains, the molecule unfolds from its inactive, “closed” conformation to expose ligand binding sites (3, 6-9). Active alphaL beta2 binds ICAM-1/CD54, ICAM-2, ICAM‑3 and JAM‑A (1, 10-12). The adhesion stabilizes interactions between T cells and antigen-presenting cells, decreases the T cell activation threshold, and facilitates leukocyte transendothelial migration to sites of inflammation (12-14). A constitutively active construct severely impairs immune responses, demonstrating that both activation and de-activation are important (14). Mutations of beta2, especially in the vWFA domain, cause leukocyte adhesion deficiency (LAD-1) and susceptibility to bacterial infections (15). The 1088 amino acid (aa) human alphaL/CD11b ECD is 73-74% aa identical to mouse and rat, and 79-80% aa identical to bovine, porcine, ovine, goat and canine alphaL ECD. A second human alphaL isoform has 53 aa inserted after aa 954 in the ECD. The 678 aa human beta2/CD18 ECD shares 81-83% aa sequence identity with mouse, rat, bovine, canine, goat, ovine, and porcine beta2 ECD.
References
- Larson, R.S. et al. (1989) J. Cell Biol. 108:703.
- Kishimoto, T.K. et al. (1987) Cell 48:681.
- Hynes, R.O. (2002) Cell 110:673.
- Qu, A. and D.J. Leavy (1995) Proc. Natl. Acad. Sci. USA 92:10277.
- Shi, M. et al. (2005) J. Biol. Chem. 280:30586.
- Beglova, N. et al. (2002) Nat. Struct. Biol. 9:282.
- Shimaoka, M. et al. (2003) Immunity 19:391.
- Lu, C. et al. (2004) J. Immunol. 173:3972.
- Cairo, C.W. et al. (2006) Immunity 25:297.
- Nortamo, P. et al. (1991) J. Immunol. 146:2530.
- de Fougerolles, A.R. and T.A. Springer (1992) J. Exp. Med. 175:185.
- Ostermann, G. et al. (2002) Nat. Immunol. 3:151.
- Heit, B. et al. (2005) J. Cell Sci. 118:5205.
- Semmrich, M. et al. (2005) J. Exp. Med. 201:1987.
- Kishimoto, T.K. et al. (1987) Cell 50:193.
Entrez Gene IDs
3683 (Human)
Gene Symbol
ITGAL
Product Documents for Recombinant Human Integrin alpha L beta 2 Protein, CF
Product Specific Notices for Recombinant Human Integrin alpha L beta 2 Protein, CF
For research use only
Citations for Recombinant Human Integrin alpha L beta 2 Protein, CF (5)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Integrin alpha L beta 2 Protein, CF include:
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Species: Human
Sample Types: Whole Cells
Applications: ELISA CapturePK Myint et al. (2021-03-08), Irisin supports integrin-mediated cell adhesion of lymphocytes Biochemistry and Biophysics Reports, 2021-026(0):100977.
PMID: 33732908 -
Species: Human
Sample Types: Protein
Applications: BioassayF Irie et al. (2021-02-26), The Cell Surface Hyaluronidase TMEM2 Regulates Cell Adhesion and Migration via Degradation of Hyaluronan at Focal Adhesion Sites The Journal of Biological Chemistry, 2021-00(0):100481.
PMID: 33647313 -
Sample Types: Protein
Applications: BioassayEC Skoog et al. (2018-05-15), CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is Associated with Alterations in Integrin Binding MBio, 2018-09(3).
PMID: 29764950 -
Applications: BioassayChakraborty S et al. (2014-07-17), FRET based quantification and screening technology platform for the interactions of leukocyte function-associated antigen-1 (LFA-1) with intercellular adhesion molecule-1 (ICAM-1). PLoS ONE, 2014-09(7):e102572.
PMID: 25032811 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayArthos J et al. (2008-02-10), HIV-1 envelope protein binds to and signals through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells. Nat. Immunol., 2008-09(3):301-9.
PMID: 18264102
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FAQs for Recombinant Human Integrin alpha L beta 2 Protein, CF
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Q: What is the amino acid sequence of the acidic and basic tails?
A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
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