Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed)

Name: Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed)
Brand: R&D Systems
SKU: 6987-GL
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 6987-GL

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Key Product Details

Source:	CHO
Accession #:	Q9UNG2.3
Structure / Form:	Non-covalently-linked trimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed)

Source

Chinese Hamster Ovary cell line, CHO-derived human GITR Ligand/TNFSF18 protein

Hemagglutinin Tag (YPYDVPDYA)	GCN4-IZ	GGGSGGGSGGGS	Human GITR Ligand (Glu52-Ser177) Accession # Q9UNG2.3
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Tyr

Predicted Molecular Mass

19.6 kDa (monomer)

SDS-PAGE

21-30 kDa, reducing conditions

Activity

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human GITR. Wyzgol, A. et al. (2009) J. Immunol. 183:1851. The ED₅₀ for this effect is 4-20 ng/mL. The activity can be enhanced approximately 10-fold in the presence of
Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

6987-GL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6987-GL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GITR Ligand/TNFSF18

GITR Ligand, also known as TNFSF18 and TL6, is an approximately 30 kDa type II transmembrane glycoprotein in the TNF superfamily (1). Human GITR Ligand consists of a 50 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 128 aa extracellular domain (ECD) (2, 3). Within the ECD, human GITR Ligand shares 56% and 60% aa sequence identity with mouse and rat GITR Ligand, respectively. GITR Ligand is expressed on antigen presenting cells,
CD4^-CD8^- double negative thymic precursors, vascular endothelial cells, neurons, and in the eye (4-11). Its expression is transiently up‑regulated by proinflammatory stimulation (4, 8, 11). The binding of GITR Ligand to GITR on mouse CD25⁺ Treg cells permits the reactivation of T cells from Treg-induced suppression, although this does not appear to occur in humans (5, 9, 12-14). GITR Ligand binding to GITR additionally provides a co‑stimulatory signal to activated CD4⁺ and CD8⁺ T cells and NK cells (5, 6, 15, 16). This interaction also induces reverse signaling in GITR Ligand expressing dendritic cells to suppress cellular activation through the same pathway induced by the immunosuppressant dexamethasone (17). In the brain, GITR Ligand/GITR interactions enhance NGF-mediated neurite outgrowth from sympathetic neurons (10).

Item	Value
References	Azuma, M. (2010) Crit. Rev. Immunol. 30:547. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056. Gurney, A.L. et al. (1999) Curr. Biol. 9:215. Stephens, G.L. et al. (2004) J. Immunol. 173:5008. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. 100:15059. Hanabuchi, S. et al. (2006) Blood 107:3617. Kamimura, Y. et al. (2009) J. Immunol. 182:2708. Hwang, H. et al. (2010) J. Neurosci. Res. 88:2188. Tuyaerts, S. et al. (2007) J. Leukoc. Biol. 82:93. O’Keefe, G.W. et al. (2008) Nat. Neurosci. 11:135. Kim, B.J. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:3170. Shimizu, J. et al. (2002) Nat. Immunol. 3:135. Ji, H. et al. (2004) J. Immunol. 172:5823. McHugh, R.S. et al. (2002) Immunity 16:311. Kanamaru, F. et al. (2004) J. Immunol. 172:7306. Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613. Grohmann, U. et al. (2007) Nat. Med. 13:579.
Long Name	Glucocorticoid Induced TNF Receptor Family Related Gene Ligand
Entrez Gene IDs	8995 (Human); 240873 (Mouse); 364031 (Rat)
Alternate Names	AITRL, AITRLMGC138237, Activation-inducible TNF-related ligand, GITR Ligand, GITRL, GITRLglucocorticoid-induced TNFR-related protein ligand, Glucocorticoid-induced TNF-related ligand, TL6AITR ligand, TNFSF18, hGITRLGITR ligand, tumor necrosis factor (ligand) superfamily, member 18, tumor necrosis factor ligand superfamily member 18

Citations for Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed) (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed) include:

Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR

AW Clarke et al. (2018), An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 0(0):1-43.
PMID: 29436901

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Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed)

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Application: In vitro bioactivity in cell culture

Verified Customer | Posted 12/06/2017

Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed)

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed)

Formulation, Preparation and Storage

Carrier Free

6987-GL

Carrier

6987-GL/CF

Carrier Free

Reconstitution Calculator

Background: GITR Ligand/TNFSF18

Citations for Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed) (1)

Customer Reviews for Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed) (1)

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Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed)

Product Documents for Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed)

Product Datasheets

COA

Certificate of Analysis

SDS

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