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Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) Best Seller

Catalog # 287-TC | R&D Systems, Inc. a Bio-Techne Brand
Analyzed by SEC-MALS
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287-TC-500

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Erythropoietin/EPO protein
Ala28-Arg193

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

21 kDa

SDS-PAGE

37 kDa, reducing conditions

Activity

Calibrated against the second international reference preparation of erythropoietin. Annable, L. et al. (1972) Bull. Hlth. Org. 47:99.

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.015-0.075 units/mL.

Scientific Data Images for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)

Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) Protein SEC-MALS.

Recombinant human EPO (Catalog # 287-TC) has a molecular weight (MW) of 28.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Graph showing dose-dependent bioactivity of Human Erythropoietin / EPO Protein

Bioactivity of Erythropoietin / EPO Protein

Recombinant Human Erythropoietin / EPO (287-TC) stimulates cell proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner. The ED50 for this effect is 0.015-0.075 units/mL.

Formulation, Preparation and Storage

287-TC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution
Reconstitute at 500 U/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt at -20 to -70 °C as supplied.
  • 1 month at 2-8 °C under sterile conditions after reconstitution.
  • 3 months at -20 to -70 °C under sterile conditions after reconstitution.

Background: Erythropoietin/EPO

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of the EPO protein is required for biological activities in vivo (4). The mature human EPO protein shares 75% - 84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the erythropoietin receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce erythropoietin production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

References

  1. Koury, M.J. (2005) Exp. Hematol. 33:1263.
  2. Jacobs, K. et al. (1985) Nature 313:806.
  3. Wen, D. et al. (1993) Blood 82:1507.
  4. Tsuda E., et al. (1990) Eur. J. Biochem. 188:405.
  5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
  6. Eckardt, K.U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
  7. Sharples, E.J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
  8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
  9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
  10. Acs, G. et al. (2001) Cancer Res. 61:3561.
  11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
  12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
  13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.

Alternate Names

ECYT5, EPO, MVCD2

Entrez Gene IDs

2056 (Human); 13856 (Mouse); 24335 (Rat)

Gene Symbol

EPO

Product Documents for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)

For research use only

Citations for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (71)

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Customer Reviews for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (2)

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  • Recombinant Human Erythropoietin (Tissue Culture Grade)
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 04/13/2018
    Using DRAQ-5 dye and GpA (erythroid cell marker) to determine levels of enucleation of differentiated cells in the Day 18 cultures. A large percentage of the cells have differentiated into mature reticulocytes/erythrocytes.
    Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) 287-TC
  • Recombinant Human Erythropoietin (Tissue Culture Grade)
    Name: Leslie Priddy
    Application: Cell migration/motility
    Verified Customer | Posted 04/13/2018

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FAQs for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)

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  • Q: Recombinant Human EPO is listed in units. How does this translate to mass?

    A: One unit of Recombinant Human EPO (Ultra Pure; Catalog # 286-EP) or Recombinant Human EPO (Tissue Culture Grade; Catalog # 287-TC) has a mass of approximately 10 ng.

  • Q: How does 287-TC differ from other Recombinant Human Erythropoietin/EPO proteins?

    A: 287-TC is considered Tissue Culture Grade. This product is tested for its ability to support the proliferation assay using TF‑1 human erythroleukemic cells. If additional testing specifications are required, the 11264-TC product is recommended. 287-TC is an excellent economical alternative intended for customers using large quantities of EPO for cultures.

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