Product Specifications for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)
Chinese Hamster Ovary cell line, CHO-derived human Erythropoietin/EPO protein Ala28-Arg193
<0.10 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
37 kDa, reducing conditions
Calibrated against the second international reference preparation of erythropoietin. Annable, L. et al. (1972) Bull. Hlth. Org. 47:99. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.015-0.075 units/mL.
Scientific Data Examples for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)
Bioactivity of Erythropoietin / EPO Protein
Recombinant Human Erythropoietin / EPO (287-TC) stimulates cell proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner. The ED50 for this effect is 0.015-0.075 units/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitute at 500 U/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 12 months from date of receipt at -20 to -70 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -70 °C under sterile conditions after reconstitution.
Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of the EPO protein is required for biological activities in vivo (4). The mature human EPO protein shares 75% - 84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the erythropoietin receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce erythropoietin production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).
Koury, M.J. (2005) Exp. Hematol. 33:1263.
Jacobs, K. et al. (1985) Nature 313:806.
Wen, D. et al. (1993) Blood 82:1507.
Tsuda E., et al. (1990) Eur. J. Biochem. 188:405.
Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
Eckardt, K.U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
Sharples, E.J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
Acs, G. et al. (2001) Cancer Res. 61:3561.
Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
Customer Reviews for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (2)
5 out of 5
2 customer ratings
Recombinant Human Erythropoietin (Tissue Culture Grade)
Reason for Rating:
Using EPO as the primary cytokine to differentiate CD34 cells to mature erythrocytes/red blood cells in an 18 day culture
Verified Customer | Posted
Using DRAQ-5 dye and GpA (erythroid cell marker) to determine levels of enucleation of differentiated cells in the Day 18 cultures. A large percentage of the cells have differentiated into mature reticulocytes/erythrocytes.
Recombinant Human Erythropoietin (Tissue Culture Grade)
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