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Recombinant Human ECE-2 Protein, CF

Catalog # 1645-ZN | R&D Systems, Inc. a Bio-Techne Brand
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1645-ZN-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ECE-2 protein
Gly104-Trp787, with an N-terminal 10-His tag and Val

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

79 kDa

SDS-PAGE

113 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is > 250 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1645-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl2.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ECE-2

ECE-2 is a zinc protease of the neprilysin (NEP) family, which also includes ECE-1, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-2 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Three alternatively spliced forms differ in their cytoplasmic tail (2). In addition to big endothelin-1, ECE-2 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, substance P, dynorphin B, proenkephalin-derived peptides such as peptide E, BAM 22 and BAM18, and PEN-LEN, an endogenous inhibitor of prohormone convertase 1 (3). Together with ECE-1, it is also involved in degradation of beta-amyloid peptide (4). The ectodomain of human ECE-2 was expressed with an N-terminal His tag and purified.

References

  1. Turner, A.J. et al. (2001) BioEssays 23:261.
  2. Lorenzo, M.N. et al. (2001) Biochim. Biophys. Acta 1522:46.
  3. Mzhavia, N. et al. (2003) J. Biol. Chem. 278:14704.
  4. Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.

Long Name

Endothelin-converting Enzyme-2

Alternate Names

ECE2

Entrez Gene IDs

9718 (Human)

Gene Symbol

ECE2

UniProt

Additional ECE-2 Products

Product Documents for Recombinant Human ECE-2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ECE-2 Protein, CF

For research use only

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