Recombinant Human DLL4 Fc Chimera Protein, CF New

Name: Recombinant Human DLL4 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: BT-DLL4

R&D Systems, part of Bio-Techne | Catalog # BT-DLL4

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Product Datasheet / COA / SDS

Analyzed by SEC-MALS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Source:	CHO
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human DLL4 Fc Chimera Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human DLL4 Fc Chimera Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human DLL4 protein

Human DLL4 (Ser27-Pro524) Accession # Q9NR61.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser 27

Predicted Molecular Mass

81 kDa

SDS-PAGE

81 kDa

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human DLL4 Fc Chimera (Catalog # BT-DLL4) binds to Recombinant Human Notch-1 Fc Chimera (Catalog # 3647-TK) with an ED₅₀ of 2.00-20.0 ng/mL.

Scientific Data Examples for Recombinant Human DLL4 Fc Chimera Protein, CF

	Recombinant Human DLL4 Fc Chimera Protein SEC-MALS. Recombinant Human DLL4 Fc Chimera Protein (Catalog # BT-DLL4) has a molecular weight (MW) of 168-185 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
	Equivalent Binding Activity of GMP and RUO grades of Recombinant Human DLL4. Equivalent binding activity of GMP (BT-DLL4-GMP) and RUO (Catalog # BT-DLL4) grades of RecombinantHuman DLL4 as measured in a functional ELISA with Recombinant Human Notch-1 Protein (orange and red, respectively).
	Recombinant Human DLL4 Fc Chimera Protein Binding Activity. In a functional ELISA, Recombinant Human DLL4 Fc Chimera Protein (Catalog # BT-DLL4) binds to Recombinant Human Notch-1 Fc Chimera (3647-TK) with an ED₅₀ of 2.00-20.0 ng/mL.
	Recombinant Human DLL4 Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Human DLL4 Fc Chimera Protein (Catalog # BT-DLL4) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 89-98 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-DLL4

Formulation	Lyophilized from a 0.2 μm filtered solution in HBS and Tween®-80 with Trehalose.
Reconstitution	Reconstitute the 20 μg size at 100 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1). The use of DLL4, in conjunction with VCAM1, has been shown as an effective method for generating hematopoietic progenitors and T cells from hPSCs in a serum- and feeder-free environment.

Item	Value
References	Shutter, J.R. et al. (2000) Genes Dev. 14:1313. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543. Walker, L. et al. (2001) Stem Cells 19:543. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.
Long Name	Delta-like 4
Entrez Gene IDs	54567 (Human); 54485 (Mouse); 100152163 (Porcine)
Alternate Names	DLL4, Delta 4 precursor, Drosophila Delta homolog 4, MGC126344, delta 4, delta ligand 4, delta-like 4 (Drosophila), delta-like 4 homolog, delta-like 4 homolog (Drosophila), delta-like 4 protein, delta-like protein 4, delta4, hdelta2, notch ligand DLL4, notch ligand delta-2

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Recombinant Human DLL4 Fc Chimera Protein, CF New

R&D Systems, part of Bio-Techne | Catalog # BT-DLL4

Key Product Details

Product Specifications for Recombinant Human DLL4 Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human DLL4 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: DLL4

Customer Reviews for Recombinant Human DLL4 Fc Chimera Protein, CF

Product Documents for Recombinant Human DLL4 Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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