Recombinant Human DLL4 Fc Chimera GMP Protein, CF GMP

R&D Systems, part of Bio-Techne | Catalog # BT-DLL4-GMP

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BT-DLL4-GMP-01M
BT-DLL4-GMP-050
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Key Product Details

  • Lot-to-lot consistency
  • Stringent guidelines for patient safety
  • Scalability necessary to support successful therapeutics
  • Test it in your process! Request a sample of GMP DLL4
  • Preclinical option available BT-DLL4

Source

CHO

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all DLL4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human DLL4 protein
Human DLL4
(Ser27-Pro524)
Accession # Q9NR61.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27-Gly-Val-Phe-Gln-Leu-Gln-Leu-Gln-Glu

Predicted Molecular Mass

81 kDa

SDS-PAGE

89-98 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human DLL4 Fc Chimera GMP (Catalog # BT-DLL4-GMP) binds to Recombinant Human Notch-1 Fc Chimera (Catalog # 3647-TK) with an ED50 of 2.00-20.0 ng/mL.

Host Cell Protein

<0.500 ng per μg of protein when tested by ELISA.

Mycoplasma

Negative for Mycoplasma.

Host Cell DNA

<0.00150 ng per µg of protein when tested by PCR.

Scientific Data Images for Recombinant Human DLL4 Fc Chimera GMP Protein, CF

Recombinant Human DLL4 GMP Protein Binding Activity.

The binding activity of Recombinant Human DLL4 GMP Protein (Catalog # BT-DLL4-GMP) was measured in a functional ELISA with Recombinant Human Notch-1 Protein. Three independent lots were tested for binding activity and plotted on the same graph to show lot-to-lot consistency of GMP DLL4 protein.

Equivalent Binding Activity ofGMP and RUO grades ofRecombinant Human DLL4

Equivalent binding activity of GMP (Catalog # BT-DLL4-GMP) and RUO (BT-DLL4) grades of Recombinant HumanDLL4 as measured in a functional ELISA with Recombinant Human Notch-1 Protein (orange and red, respectively).

Recombinant Human DLL4 Fc Chimera GMP Protein SDS-PAGE.

2 μg/lane of Recombinant Human DLL4 Fc Chimera GMP Protein (Catalog # BT-DLL4-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 89-98 kDa and 180-200 kDa, respectively.

Formulation, Preparation and Storage

BT-DLL4-GMP
Formulation Lyophilized from a 0.2 μm filtered solution in HBS and Tween®-80 with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in sterile water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. DLL-4 is the only Notch ligand expressed predominantly by the vascular endothelium,  making it critical in vascular development.  The mature extracellular domain (ECD) of human DLL4 shares 86% amino acid sequence identity with mouse DLL4.  The use of DLL4, in conjunction with VCAM1, has been shown as an effective method for generating hematopoietic progenitors and T cells from hPSCs in a serum- and feeder-free environment.

Long Name

Delta-like 4

Alternate Names

delta 4, Delta 4 precursor, delta ligand 4, delta4, delta-like 4 (Drosophila), delta-like 4 homolog, delta-like 4 homolog (Drosophila), delta-like 4 protein, delta-like protein 4, Drosophila Delta homolog 4, hdelta2, MGC126344, notch ligand delta-2, notch ligand DLL4

Entrez Gene IDs

54567 (Human); 54485 (Mouse); 100152163 (Porcine)

Gene Symbol

DLL4

Additional DLL4 Products

Product Documents for Recombinant Human DLL4 Fc Chimera GMP Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

  • Designed, manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
  • Documented and controlled manufacturing process
  • Control of documentation and process changes by QA
  • Personnel training programs
  • Raw material inspection and vendor qualification/monitoring program
  • Validated equipment, processes and test methods
  • Equipment calibration and maintenance schedules using a Regulatory Asset Manager
  • Facility/Utilities maintenance, contamination controls, safety and pest control programs
  • Material review process for variances
  • Robust product stability program following relevant ICH guidelines

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their application. Each product is provided with a lot-specific Certificate of Analysis that contains the product's specifications and test results. Quality control testing may include, but is not limited to:

  • N-terminal amino acid analysis
  • SDS-PAGE purity analysis
  • Molecular weight analysis via mass spectrometry
  • Endotoxin assessment per USP <85> and Ph. Eur. 2.6.14 guidelines
  • Bioassay analysis
  • Microbial testing per USP <71> and Ph. Eur. 2.6.1 guidelines
  • Host cell protein assessment
  • Host cell DNA assessment
  • Mycoplasma assessment

Additional testing and documentation requested by the customer can be arranged at an additional cost. 

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis and St. Paul, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.

Product Specific Notices for Recombinant Human DLL4 Fc Chimera GMP Protein, CF

Full terms and conditions of sale can be found online in the Protein Sciences Segment T&Cs at: Terms & Conditions.

For preclinical, or clinical ex vivo use

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