Recombinant Human Notch-1 Fc Chimera Protein, CF

Name: Recombinant Human Notch-1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 3647-TK
Rating: 4 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3647-TK

Citations (4)

(3)

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Key Product Details

Source:	NS0
Accession #:	P46531
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Human Notch-1 Fc Chimera Protein, CF

Product Specifications for Recombinant Human Notch-1 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human Notch-1 protein

Human Notch-1 (Ala19 - Gln526) Accession # P46531	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala19

Predicted Molecular Mass

80.1 kDa (monomer)

SDS-PAGE

115-125 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized rhNotch-1 Fc Chimera at 5 µg/mL (100 µL/well) can bind rrJagged-1/Fc Chimera with an apparent K_D<5 nM in a functional ELISA.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3647-TK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Notch-1

Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes (1 - 3). Notch signaling is important for maintaining stem cells and inducing differentiation, especially in the nervous system and lymphoid tissues (2 - 4). Notch can specify binary cell fates; for example, promoting T- over B-cell development from a common precursor (2). More than 50% of human T-lineage acute lymphoblastic leukemia (T-ALL) have activating mutations of Notch1 (1, 5). Human Notch-1 is synthesized as a 2556 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1718 aa extracellular domain (ECD) with 36 EGF-like repeats and three Lin-12/Notch repeats (LNR), a 23 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain containing six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The 11^th and 12^th EGF-like repeats bind ligands including Jagged and Delta-like families in humans (6). O-fucosylation by Fringe family members at a site within this region can inhibit the interaction of Notch with Jagged ligands, thereby promoting Delta-like ligand interactions (7). Notch-1 receptor undergoes post-translational furin-type proteolytic cleavage, forming a heterodimer through interaction of a hydrophobic area C-terminal to the LNR on the 1647 aa ligand-binding extracellular region with the 891 aa transmembrane/cytoplasmic portion (8, 9). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM-17) and the presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (10). Human Notch-1 ECD aa 19 - 526, including the first 13 EGF repeats, shows 91% aa identity with corresponding regions of mouse and rat, 89% with canine, and 79% with chicken Notch-1. This region also exhibits 60% aa identity with human Notch-2 and Notch-3.

Item	Value
References	Ellisen, L. W. et al. (1991) Cell 66:649. Dumortier, A. et al. (2005) Int. J. Hematol. 82:277. Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709. Androutsellis-Theotokis, A. et al. (2006) Nature 442:823. Weng, A. P. et al. (2004) Science 306:269. Hambleton, S. et al. (2004) Structure 12:2173. Yang, L. et al. (2005) Mol. Biol. Cell 16:927. Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265. Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
Entrez Gene IDs	4851 (Human); 25496 (Rat)
Alternate Names	EC 2.1.2.11, EC 3.4.21.68, Notch (Drosophila) homolog 1 (translocation-associated), Notch homolog 1, translocation-associated, Notch-1, Notch1, TAN1, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1, hN1, notch 1Notch homolog 1, translocation-associated (Drosophila)

Citations for Recombinant Human Notch-1 Fc Chimera Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Notch-1 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

BV Maruthacha et al. (2022), Generation of synthetic antibody fragments with optimal complementarity determining region lengths for Notch-1 recognition Frontiers in Microbiology, 13(0):931307.
PMID: 35992693
Applications: ELISA (Standard)

Y Li et al. (2018), ABT-165, a Dual Variable Domain Immunoglobulin (DVD-Ig) Targeting DLL4 and VEGF, Demonstrates Superior Efficacy and Favorable Safety Profiles in Preclinical Models Mol. Cancer Ther., 0(0).
PMID: 29592882
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Andrawes M et al. (2013), Intrinsic selectivity of Notch 1 for Delta-like 4 over Delta-like 1. J Biol Chem, 288(35):25477-89.
PMID: 23839946
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Scehnet JS et al. (2007), Inhibition of Dll4-mediated signaling induces proliferation of immature vessels and results in poor tissue perfusion. Blood, 109(11):4753-60.
PMID: 17311993

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Customer Reviews for Recombinant Human Notch-1 Fc Chimera Protein, CF (3)

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Name: Jignesh Ganeshbhai Patel

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 09/10/2018
Name: Jignesh Patel

Application: Enzymatic activity in vitro

Verified Customer | Posted 09/10/2018
Name: Jignesh Patel

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 08/21/2018

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Product Documents for Recombinant Human Notch-1 Fc Chimera Protein, CF

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