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Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein, CF

Catalog # 10364-DC | R&D Systems, Inc. a Bio-Techne Brand
HEK293 Expressed
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10364-DC-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Dectin-1/CLEC7A protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human Dectin-1/CLEC7A
(Thr66-Met247)
Accession # Q9BXN2.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met Asp-Pro100

Predicted Molecular Mass

47 kDa

SDS-PAGE

54-64 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Dectin-1/CLEC7A Fc Chimera (Catalog # 10364-DC) is immobilized at 0.25 µg/mL (100 µL/well), Biotinylated Laminarin (1, 3-beta-glucan) binds with an ED50 of 0.08-9.6 ng/mL.

Scientific Data Images for Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein, CF

Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein Binding Activity

Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein Binding Activity

When Recombinant Human Dectin-1/CLEC7A Fc Chimera (Catalog # 10364-DC) is immobilized at 0.25 μg/mL (100 μL/well), Biotinylated Laminarin (1, 3-beta-glucan) binds with an ED50 of 0.08-9.6 ng/mL.
Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein SDS-PAGE

Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human Dectin-1/CLEC7A Fc Chimera (Catalog # 10364-DC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-64 kDa and 108-128 kDa, respectively.

Formulation, Preparation and Storage

10364-DC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta-glucan receptor, is a type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (ECD) (3, 4). Alternate splicing generates one major isoform expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells that lacks the stalk region (3-6). The mature ECD of human Dectin-1 shares 59% and 57% amino acid (aa) sequence identity with mouse and rat Dectin-1, respectively. The CRD of Dectin-1 selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5-7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8-10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15, 16).

References

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Alternate Names

CD369, CLEC7A, CLECSF12, Dectin1

Entrez Gene IDs

64581 (Human); 56644 (Mouse)

Gene Symbol

CLEC7A

UniProt

Product Documents for Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein, CF

For research use only

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