Recombinant Human CX3CL1/Fractalkine (Chemokine Domain)

Name: Recombinant Human CX3CL1/Fractalkine (Chemokine Domain)
Brand: R&D Systems
SKU: 362-CX
Price: 377 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 362-CX

Citations (9)

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Source:	E. coli
Accession #:	Q6I9S9
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CX3CL1/Fractalkine (Chemokine Domain)

Product Specifications for Recombinant Human CX3CL1/Fractalkine (Chemokine Domain)

Source	E. coli-derived human CX3CL1/Fractalkine protein Gln25-Gly100
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gln25
Predicted Molecular Mass	8.5 kDa
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED₅₀ for this effect is 2‑10 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CX3CR1. The ED₅₀ for this effect is 0.3-1.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

362-CX

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

362-CX/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CX3CL1/Fractalkine

CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X₃-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes. The gene for human CX3CL1 has been mapped to chromosome 16q.

Item	Value
References	Pan, Y. et al. (1997) Nature 387:611. Bazan, J.F. et al. (1997) Nature 385:640. Mackay, C.R. (1997) Current Biology 7:R384.
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names	ABCD-3, C3Xkine, CX3C membrane-anchored chemokine, CX3CL1, CXC3, CXC3C, FKN, Fractalkine, NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, NTTSmall-inducible cytokine D1, Neurotactin, SCYD1C-X3-C motif chemokine 1, chemokine (C-X3-C motif) ligand 1, neurotactin), small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Citations for Recombinant Human CX3CL1/Fractalkine (Chemokine Domain) (9)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CX3CL1/Fractalkine (Chemokine Domain) include:

AL Collar et al. (2018), The homozygous CX3CR1-M280 mutation impairs human monocyte survival JCI Insight, 3(3).
PMID: 29415879
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Park et al. (2017), Fractalkine induces angiogenic potential in CX3CR1-expressing monocytes J. Leukoc. Biol., 0(0).
PMID: 28978662
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Plasmodium falciparum proteins involved in cytoadherence of infected erythrocytes to chemokine CX3CL1 Sci Rep, 6(0):33786.
PMID: 27653778
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Truman LA et al. (2008), CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis. Blood, 112(13):5026-36.
PMID: 18799722
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Holmes FE et al. (2008), Intra-neural administration of fractalkine attenuates neuropathic pain-related behaviour. J. Neurochem., 106(2):640-9.
PMID: 18410510
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Clark AK et al. (2007), Inhibition of spinal microglial cathepsin S for the reversal of neuropathic pain. Proc. Natl. Acad. Sci. U.S.A., 104(25):10655-60.
PMID: 17551020
Species: Human

Applications: Control

Latchney LR et al. (2004), Immunohistochemical assessment of fractalkine, inflammatory cells, and human herpesvirus 7 in human salivary glands. J. Histochem. Cytochem., 52(5):671-81.
PMID: 15100244
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Deiva K et al. (2004), Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation. Eur. J. Neurosci., 20(12):3222-32.
PMID: 15610155
Species: N/A
Sample Types: N/A
Applications: Antibody Absorption

Lucas AD et al. (2001), The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo. Am. J. Pathol., 158(3):855-66.
PMID: 11238035

Recombinant Human CX3CL1/Fractalkine (Chemokine Domain)

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human CX3CL1/Fractalkine (Chemokine Domain)

Formulation, Preparation and Storage

Carrier Free

362-CX

Carrier

362-CX/CF

Carrier Free

Reconstitution Calculator

Background: CX3CL1/Fractalkine

Citations for Recombinant Human CX3CL1/Fractalkine (Chemokine Domain) (9)

Product Documents for Recombinant Human CX3CL1/Fractalkine (Chemokine Domain)

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SDS

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