Recombinant Human CTLA-4 Fc Chimera His-tag Protein

Name: Recombinant Human CTLA-4 Fc Chimera His-tag Protein
Brand: R&D Systems
SKU: 325-CT
Rating: 5 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 325-CT

Citations (7)

(4)

Product Datasheet / COA / SDS

New Version Available - 7268-CT

New rh CTLA-4 Available! Increased activity; Enhanced purity; Lower endotoxin specification; CHO expressed!

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Setting the standard in quality research reagents

Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	Q6GR94
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human CTLA-4 Fc Chimera His-tag Protein

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CTLA-4 protein

Human CTLA-4 (Ala37-Phe162) Accession # Q6GR94	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala37

Predicted Molecular Mass

40 kDa (monomer)

Activity

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. Linsley, P.S. et al. (1991) J. Exp. Med. 174:561. The ED₅₀ for this effect is 0.1-0.4 µg/mL when stimulated with 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog #
140-B1) in the presence of PHA.

Scientific Data Examples for Recombinant Human CTLA-4 Fc Chimera His-tag Protein

	Recombinant Human CTLA-4 Fc Chimera His-tag Protein Bioactivity Recombinant Human CTLA-4 Fc Chimera (Catalog # 325-CT) inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 0.1-0.4 µg/mL when stimulated with 1 µg/mL Recombinant Human B7-1 Fc Chimera (Catalog # 140-B1) in the presence of PHA.
	Recombinant Human CTLA-4 Fc Chimera His-tag Protein SDS-PAGE 1 μg/lane of Recombinant Human CTLA-4 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 51 kDa and 94 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

325-CT

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

325-CT/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CTLA-4

CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily (1, 2). Human or mouse CTLA-4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence. It is found as a covalent homodimer of 41‑43 kDa (2). Within the ECD, human CTLA-4 shares 68%, 71% and 83‑86% aa sequence identity with mouse, rat and porcine/bovine/rabbit/feline/canine CTLA-4, respectively. A 174 aa form that lacks TM and cytoplasmic sequences (sCTLA-4) is possibly secreted (3‑5). Isoforms of 56‑79 aa that mainly contain parts of the cytoplasmic domain are reported. In mouse, an isoform lacking the Ig-like domain has ligand-independent inhibitory activity and is termed liCTLA-4 (6). CD28, which is structurally related to CTLA-4, is constitutively expressed on naïve T cells and promotes T cell activation when engaged by B7-2 on antigen-presenting cells (APC) within the immunological synapse (IS) (1, 7, 8). In contrast, CTLA-4 is recruited from intracellular vesicles to the IS beginning 1, 2 days after T cell activation (2, 7, 8). It forms a linear lattice with B7‑1 on APC, inducing negative regulatory signals and ending T cell activation (9). Abatacept, a therapeutic human CTLA-4-Ig fusion protein (trade name Orencia), competes with CD28 for B7-1 and B7-2 binding and has been used to antagonize T cell activation in autoimmune conditions and to enhance transplant survival (10). Mice deleted for CTLA-4 show no abnormalities until after birth, but then develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells, which constitutively express CTLA-4 in wild-type mice and humans (11‑13).

Item	Value
References	Harper, K. et al. (1991) J. Immunol. 147:1037. Teft, W.A. et al. (2006) Annu. Rev. Immunol. 24:65. Magistrelli, G. et al. (1999) Eur. J. Immunol. 29:3596. Tector, M. et al. (2009) BMC Immunol. 10:51. Oaks, M.K. and K.M. Hallett (2000) J. Immunol. 164:5015. Vijayakrishnan, L. et al. (2004) Immunity 20:563. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401. Jansson, A. et al. (2005) J. Immunol 175:1575. Darlington, P.J. et al. (2005) J. Immunol. 175:996. Platt, A.M. et al. (2010) J. Immunol. 185:1558. Wing, K. et al. (2008) Science 322:271. Friedline, R.H. et al. (2009) J. Exp. Med. 206:421. Jain, N. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1524.
Long Name	Cytotoxic T-lymphocyte-associated Molecule 4
Entrez Gene IDs	1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)
Alternate Names	CD, CD152, CD152 antigen, CD152IDDM12, CD28, CELIAC3, CTLA-4, CTLA4, Cytotoxic T-lymphocyte-associated antigen 4, GRD4, GSE, ICOS, celiac disease 3, cytotoxic T-lymphocyte antigen 4, cytotoxic T-lymphocyte protein 4, cytotoxic T-lymphocyte-associated protein 4, cytotoxic T-lymphocyte-associated serine esterase-4, ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Citations for Recombinant Human CTLA-4 Fc Chimera His-tag Protein (7)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CTLA-4 Fc Chimera His-tag Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

BD Hock et al. (2017), Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells PLoS ONE, 12(3):e0172858.
PMID: 28257435
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kowalczyk A et al. (2014), Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3. Eur J Immunol, 44(4):1143-55.
PMID: 24338929
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Steidl C et al. (2011), MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers. Nature, 471(7338):377-81.
PMID: 21368758
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Maniar A et al. (2010), Human gammadelta T lymphocytes induce robust NK cell-mediated antitumor cytotoxicity through CD137 engagement. Blood, 116(10):1726-33.
PMID: 20519625
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Shan M et al. (2009), Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema. Sci Transl Med, 1(4):4ra10.
PMID: 20368170
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Sakthivel P et al. (2007), The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J. Intern. Med., 261(4):399-407.
PMID: 17391115
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Lazetic S et al. (2002), Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells. J. Biol. Chem., 277(41):38660-8.
PMID: 12167647

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Customer Reviews for Recombinant Human CTLA-4 Fc Chimera His-tag Protein (4)

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Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 02/11/2019

0.5 ug mCTLA-4-hIgG1 (Cat# 325-CT-200) was added to cells expressing mB7-1 GFP. Binding was detected with anti-human 594 secondary antibody.
Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 11/02/2018
Recombinant Human CTLA-4 Fc Chimera His Tagged Protein

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 03/24/2018
Recombinant Human CTLA-4 Fc Chimera His Tagged Protein

Name: Kimberly Dickinson

Application: Binding assay/Protein-protein interaction

Reason for Rating: Works in our assay great!

Verified Customer | Posted 03/22/2018

Recombinant Human CTLA-4 Fc Chimera His-tag Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

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Product Specifications for Recombinant Human CTLA-4 Fc Chimera His-tag Protein

Scientific Data Examples for Recombinant Human CTLA-4 Fc Chimera His-tag Protein

Formulation, Preparation and Storage

Carrier Free

325-CT

Carrier

325-CT/CF

Carrier Free

Reconstitution Calculator

Background: CTLA-4

Citations for Recombinant Human CTLA-4 Fc Chimera His-tag Protein (7)

Customer Reviews for Recombinant Human CTLA-4 Fc Chimera His-tag Protein (4)

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Recombinant Human CTLA-4 Fc Chimera His Tagged Protein

Recombinant Human CTLA-4 Fc Chimera His Tagged Protein

Product Documents for Recombinant Human CTLA-4 Fc Chimera His-tag Protein

Product Datasheets

COA

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SDS

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