Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

Name: Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein
Brand: R&D Systems
SKU: 7537-BF
Rating: 5 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7537-BF

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Key Product Details

Source:	CHO
Accession #:	Q9Y275
Structure / Form:	High Molecular Weight Multimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

Source	Chinese Hamster Ovary cell line, CHO-derived human BAFF/BLyS/TNFSF13B protein Ala134-Leu285
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala134
Predicted Molecular Mass	17.0 kDa (monomer)
SDS-PAGE	19 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED₅₀ for this effect is 0.2-1.2 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

7537-BF

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7537-BF/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: BAFF/BLyS/TNFSF13B

B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF. It can be expressed as a homo-oligomer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg133 and Ala134 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF co‑stimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14‑16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).

Item	Value
References	Lied, G.A. and A. Berstad (2011) Scand. J. Immunol. 73:1. Mackay, F. et al. (2010) Immunol. Rev. 237:205. Moore, P.A. et al. (1999) Science 285:260. Schneider, P. et al. (1999) J. Exp. Med. 189:1747. Roschke, V. et al. (2002) J. Immunol. 169:4314. Scapini, P. et al. (2003) J. Exp. Med. 197:297. Gavin, A.L. et al. (2003) J. Biol. Chem. 278:38220. Alexaki, V.-I. et al. (2009) J. Immunol. 183:5948. Yu, G. et al. (2000) Nat. Immunol. 1:252. Thompson, J.S. et al. (2001) Science 293:2108. Schiemann, B. et al. (2001) Science 293:2111. Litinskiy, M.B. et al. (2002) Nat. Immunol. 3:822. Batten, M. et al. (2000) J. Exp. Med. 192:1453. Huard, B. et al. (2001) J. Immunol. 167:6225. Sutherland, A.P.R. et al. (2005) J. Immunol. 174:5537. Walters, S. et al. (2009) J. Immunol. 182:793. Chang, S.K. et al. (2006) Blood 108:2687.
Long Name	B cell Activating Factor
Entrez Gene IDs	10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names	ApoL related ligand TALL-1, B lymphocyte stimulator, B-cell-activating factor, BAFF, BAFFB-cell activating factor, BLYSB-lymphocyte stimulator, BLyS, CD257, CD257 antigen, DTL, Dendritic cell-derived TNF-like molecule, TALL-1delta BAFF, TALL1, TALL1Delta4 BAFF, THANK, TNF and ApoL-related leukocyte expressed ligand 1, TNF homolog that activates apoptosis, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, ZTNF4, tumor necrosis factor (ligand) superfamily, member 13b, tumor necrosis factor (ligand) superfamily, member 20, tumor necrosis factor ligand superfamily member 13B, tumor necrosis factor superfamily, member 13b, tumor necrosis factor-like protein ZTNF4

Citations for Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

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Customer Reviews for Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein (4)

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Name: Jenna Nguyen

Application: Cell migration/motility

Verified Customer | Posted 04/05/2019
Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

Name: Jenna Nguyen

Application: Enzymatic activity in vitro

Verified Customer | Posted 11/29/2018
Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

Name: Jenna Nguyen

Application: SDS-PAGE Control

Verified Customer | Posted 11/17/2018
Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

Name: Jenna Nguyen

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 11/15/2018

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Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

Formulation, Preparation and Storage

Carrier Free

7537-BF

Carrier

7537-BF/CF

Carrier Free

Reconstitution Calculator

Background: BAFF/BLyS/TNFSF13B

Citations for Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

Customer Reviews for Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein (4)

Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

Product Documents for Recombinant Human BAFF/BLyS/TNFSF13B (CHO-expressed) Protein

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