Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Chimera Protein, CF

Name: Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10472-B3

Catalog # 10472-B3

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	HEK293
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Chimera Protein, CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human B7-H3 protein

Human B7-H3 (4Ig)/B7-H3b (Leu29-Thr461) Accession # NP_001019907.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu29

Predicted Molecular Mass

73 kDa

SDS-PAGE

100-120 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 2-20 µg/mL.

Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Chimera Protein (10472-B3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-120 kDa and 200-240 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10472-B3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Mass (in vial)

Desired Reconstitution Concentration

Background: B7-H3

Human B7 homolog 3 (B7-H3), also known as CD276, is a member of the B7 family of immune checkpoint molecules, responsible for regulating immune responses (1‑3). There are at least seven structurally related B7 family members, all sharing about 20-40% amino acid (aa) sequence identity (4). The B7 protein family are immunoglobulin (Ig) superfamily members with varying numbers of Ig-V-like and Ig-C-like regions in the extracellular domain (ECD) and they can either be glycosylphosphatidylinositol (GPI)-linked or transmembrane (4). The mature ECD of B7-H3 contains two V-like and two C-like Ig domains, a transmembrane region, and a short cytoplasmic domain. An isoform of human B7-H3 containing only a single set of V-like and C-like Ig domains in the ECD has also been identified (1, 5). The ECD of both mouse and rat B7-H3 only contain a single set of V-like and C-like Ig domains. Human B7-H3 is not expressed on resting B cells, T cells, monocytes or dendritic cells, but is induced on dendritic cells and monocytes by inflammatory cytokines (1, 6). B7-H3 is also overexpressed in numerous cancers including bladder, breast and melanoma (7). Unlike other B7 family members, human B7-H3 does not bind any known members of the CD28 family of immunoreceptors and its receptor has yet to be identified. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to costimulate the proliferation of CD4+ or CD8+ T cells (8). B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production (1-3, 8).

Item	Value
References	Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203. Collins, M. et al. (2005) Genome Biol. 6:223. Ling, V. et al. (2003) Genomics. 82:365. Prasad, D.V. et al. (2004) J Immunol. 173:2500. Dong, P. et al. (2018) Front Oncol. 8:264. Suh, W.K. et al. (2003) Nat Immunol. 4:899.
Long Name	B7 Homolog 3
Entrez Gene IDs	80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)
Alternate Names	B7-H3, B7-H3B7 homolog 3, B7H3, B7H34Ig-B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule

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