Recombinant Human B7-1/CD80 Fc Chimera Protein, CF

Name: Recombinant Human B7-1/CD80 Fc Chimera Protein, CF
Brand: rnd
SKU: 10133-B1

Catalog # 10133-B1

Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	P33681
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human B7-1/CD80 Fc Chimera Protein, CF

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human B7-1/CD80 protein

Human B7-1/CD80 (Val35-Asn242) Accession # P33681	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Val35

Predicted Molecular Mass

50.5 kDa

SDS-PAGE

66-80 kDa, under reducing conditions

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED₅₀ for this effect is 0.025‑0.15 µg/mL in the presence of PHA.

Recombinant Human B7-1/CD80 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Human B7-1/CD80 Fc Chimera Protein Bioactivity Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 10133-B1) induces IL-2 secretion by Jurkat human acute T cell leukemia cells in the presence of PHA. The ED₅₀for this effect is 0.025-0.15 μg/mL.
	Recombinant Human B7-1/CD80 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 10133-B1) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 66-80 kDa and 130-160 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10133-B1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-1/CD80

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-6). Mature human B7-1 consists of a 208 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain (7). Within the ECD, human B7-1 shares 50% aa sequence identity with mouse B7-1. Alternative splicing generates a 30 kDa soluble isoform that lacks the transmembrane segment and retains the ability to bind CD28 and CTLA-4 and an isoform that lacks the second Ig-like domain and the transmembrane segment (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2).

Item	Value
References	Ville, S. et al. (2015) Front. Immunol. 6:411. Azuma, M. et al. (1993) Nature 366:76. Freeman, G.J. et al. (1993) Science 262:909. Chen, C. et al. (1994) J. Immunol. 152:4929. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185 Lanier, L. et al. (1995) J. Immunol. 154:97. Freeman, G.J. et al. (1989) J. Immunol. 143:2714. Kakoulidou, M. et al. (2007) Scand. J. Immunol. 66:529.
Entrez Gene IDs	941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names	Activation B7-1 antigen, B7, B7-1, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 molecule, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80, costimulatory factor CD80, costimulatory molecule variant IgV-CD80

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