Recombinant Human Axl His-tag Protein, CF

Name: Recombinant Human Axl His-tag Protein, CF
Brand: rnd
SKU: 10448-AL
Rating: 5 (1 reviews)

Catalog # 10448-AL

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Key Product Details

Source:	CHO
Accession #:	P30530.4
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Axl His-tag Protein, CF

Technical Data
Citations / Reviews (1) / FAQs

Product Specifications

Source	Chinese Hamster Ovary cell line, CHO-derived human Axl protein Glu33-Trp450, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu 33
Predicted Molecular Mass	46 kDa
SDS-PAGE	74-82 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human Axl-His-tag (Catalog # 10448-AL) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human GAS6 (Catalog # 885-GSB) binds with an ED₅₀ of 0.1-0.5 ng/mL.

Recombinant Human Axl His-tag Protein, CF Scientific Data Examples

	Recombinant Human Axl His-tag Protein Binding Activity When Recombinant Human Axl His-tag (Catalog # 10448-AL) is immobilized at 0.25 µg/mL (100 µL/well), it binds to Recombinant Human Gas6 (Catalog # 885-GSB) with an ED₅₀ of 0.1-0.5 ng/mL.
	Recombinant Human Axl His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Human Axl His-tag Protein (Catalog# 10448-AL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-82 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10448-AL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Axl

Axl, also known as Ufo and Ark, is a widely expressed 140 kDa glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis (1). Mature human Axl consists of a 426 aa extracellular domain (ECD) that contains two Ig-like domains and two fibronectin type III domains, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain that includes the tyrosine kinase domain (2). Within the ECD, human Axl shares approximately 82% aa sequence identity with mouse and rat Axl. A short alternately spliced form of human Axl has a 9 aa deletion in the extracellular juxtamembrane region (2). Axl binds the vitamin K-dependent protein Gas6 which triggers tyrosine autophosphorylation of the Axl cytoplasmic domain (3). Activation of Axl induces a broad range of activities including platelet aggregation and thrombus formation (4), macrophage and dendritic cell phagocytosis of apoptotic cells (5), NK cell development from hematopoietic progenitor cells (6), and in vivo angiogenesis (7). Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness (7, 8). It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow (9). It also functions as a cellular entry receptor for Gas6-opsonized lentiviruses (10). A 70-80 kDa soluble portion of the Axl ECD can be shed by proteolytic cleavage, and this fragment retains the ability to bind Gas6 (11, 12).

Item	Value
References	Linger, R.M.A. et al. (2011) Adv. Cancer Res. 100:35. O’Bryan, J.P. et al. (1991) Mol. Cell. Biol. 11:5016. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022. Cosemans, J.M.E.M. et al. (2010) J. Thromb. Haemost. 8:1797. Seitz, H.M. et al. (2007) J. Immunol. 178:5635. Park, I.-K. et al. (2009) Blood 113:2470. Holland, S. et al. (2005) Cancer Res. 65:9294. Rankin, E.B. et al. (2010) Cancer Res. 70:7570. Korshunov, V.A. et al. (2006) Circ. Res. 98:1446. Morizono, K. et al. (2011) Cell Host Microbe 9:286. O’Bryan, J.P. et al. (1995) J. Biol. Chem. 270:551. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.
Long Name	Axl Receptor Tyrosine Kinase
Entrez Gene IDs	558 (Human); 26362 (Mouse)
Alternate Names	AI323647, ARK, AXL oncogene, AXL receptor tyrosine kinase, AXL transforming sequence/gene, Axl, EC 2.7.10, EC 2.7.10.1, JTK11, Tyro7, UFO, UFOoncogene AXL, tyrosine-protein kinase receptor UFO

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Recombinant Human Axl His-tag Protein, CF

Name: Anonymous

Application: Immunoassay Standard

Reason for Rating: Parallel with endogenous human Axl

Verified Customer | Posted 12/16/2020

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Recombinant Human Axl His-tag Protein, CF

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Key Product Details

Product Specifications

Recombinant Human Axl His-tag Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Axl

Citations

Customer Reviews (1)

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Recombinant Human Axl His-tag Protein, CF

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