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Recombinant Human Axl His-tag Protein, CF

Catalog # 10448-AL | R&D Systems, Inc. a Bio-Techne Brand
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10448-AL-100

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Axl protein
Glu33-Trp450, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu 33

Predicted Molecular Mass

46 kDa

SDS-PAGE

74-82 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Axl-His-tag (Catalog # 10448-AL) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human GAS6 (Catalog # 885-GSB) binds with an ED50 of 0.1-0.5 ng/mL.

Scientific Data Images for Recombinant Human Axl His-tag Protein, CF

Recombinant Human Axl His-tag Protein Binding Activity

Recombinant Human Axl His-tag Protein Binding Activity

When Recombinant Human Axl His-tag (Catalog # 10448-AL) is immobilized at 0.25 µg/mL (100 µL/well), it binds to Recombinant Human Gas6 (Catalog # 885-GSB) with an ED50 of 0.1-0.5 ng/mL.
Recombinant Human Axl His-tag Protein SDS-PAGE

Recombinant Human Axl His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Axl His-tag Protein (Catalog# 10448-AL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-82 kDa.

Formulation, Preparation and Storage

10448-AL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl, also known as Ufo and Ark, is a widely expressed 140 kDa glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis (1). Mature human Axl consists of a 426 aa extracellular domain (ECD) that contains two Ig-like domains and two fibronectin type III domains, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain that includes the tyrosine kinase domain (2). Within the ECD, human Axl shares approximately 82% aa sequence identity with mouse and rat Axl. A short alternately spliced form of human Axl has a 9 aa deletion in the extracellular juxtamembrane region (2). Axl binds the vitamin K-dependent protein Gas6 which triggers tyrosine autophosphorylation of the Axl cytoplasmic domain (3). Activation of Axl induces a broad range of activities including platelet aggregation and thrombus formation (4), macrophage and dendritic cell phagocytosis of apoptotic cells (5), NK cell development from hematopoietic progenitor cells (6), and in vivo angiogenesis (7). Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness (7, 8). It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow (9). It also functions as a cellular entry receptor for Gas6-opsonized lentiviruses (10). A 70-80 kDa soluble portion of the Axl ECD can be shed by proteolytic cleavage, and this fragment retains the ability to bind Gas6 (11, 12).

References

  1. Linger, R.M.A. et al. (2011) Adv. Cancer Res. 100:35.
  2. O’Bryan, J.P. et al. (1991) Mol. Cell. Biol. 11:5016.
  3. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  4. Cosemans, J.M.E.M. et al. (2010) J. Thromb. Haemost. 8:1797.
  5. Seitz, H.M. et al. (2007) J. Immunol. 178:5635.
  6. Park, I.-K. et al. (2009) Blood 113:2470.
  7. Holland, S. et al. (2005) Cancer Res. 65:9294.
  8. Rankin, E.B. et al. (2010) Cancer Res. 70:7570.
  9. Korshunov, V.A. et al. (2006) Circ. Res. 98:1446.
  10. Morizono, K. et al. (2011) Cell Host Microbe 9:286.
  11. O’Bryan, J.P. et al. (1995) J. Biol. Chem. 270:551.
  12. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.

Long Name

Axl Receptor Tyrosine Kinase

Alternate Names

AI323647, ARK, JTK11, Tyro7, UFO

Entrez Gene IDs

558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)

Gene Symbol

AXL

UniProt

Product Documents for Recombinant Human Axl His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Axl His-tag Protein, CF

For research use only

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