Product Specifications for Recombinant Human Axl His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human Axl protein Glu33-Trp450, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
74-82 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Human Axl-His-tag (Catalog # 10448-AL) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human GAS6 (Catalog # 885-GSB) binds with an ED50 of 0.1-0.5 ng/mL.
Scientific Data Examples for Recombinant Human Axl His-tag Protein, CF
Recombinant Human Axl His-tag Protein Binding Activity
When Recombinant Human Axl His-tag (Catalog # 10448-AL) is immobilized at 0.25 µg/mL (100 µL/well), it binds to Recombinant Human Gas6 (Catalog # 885-GSB) with an ED50 of 0.1-0.5 ng/mL.
Recombinant Human Axl His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human Axl His-tag Protein (Catalog# 10448-AL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-82 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 1 mg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Axl, also known as Ufo and Ark, is a widely expressed 140 kDa glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis (1). Mature human Axl consists of a 426 aa extracellular domain (ECD) that contains two Ig-like domains and two fibronectin type III domains, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain that includes the tyrosine kinase domain (2). Within the ECD, human Axl shares approximately 82% aa sequence identity with mouse and rat Axl. A short alternately spliced form of human Axl has a 9 aa deletion in the extracellular juxtamembrane region (2). Axl binds the vitamin K-dependent protein Gas6 which triggers tyrosine autophosphorylation of the Axl cytoplasmic domain (3). Activation of Axl induces a broad range of activities including platelet aggregation and thrombus formation (4), macrophage and dendritic cell phagocytosis of apoptotic cells (5), NK cell development from hematopoietic progenitor cells (6), and in vivo angiogenesis (7). Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness (7, 8). It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow (9). It also functions as a cellular entry receptor for Gas6-opsonized lentiviruses (10). A 70-80 kDa soluble portion of the Axl ECD can be shed by proteolytic cleavage, and this fragment retains the ability to bind Gas6 (11, 12).
Linger, R.M.A. et al. (2011) Adv. Cancer Res. 100:35.
O’Bryan, J.P. et al. (1991) Mol. Cell. Biol. 11:5016.
Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
Cosemans, J.M.E.M. et al. (2010) J. Thromb. Haemost. 8:1797.
Seitz, H.M. et al. (2007) J. Immunol. 178:5635.
Park, I.-K. et al. (2009) Blood 113:2470.
Holland, S. et al. (2005) Cancer Res. 65:9294.
Rankin, E.B. et al. (2010) Cancer Res. 70:7570.
Korshunov, V.A. et al. (2006) Circ. Res. 98:1446.
Morizono, K. et al. (2011) Cell Host Microbe 9:286.
O’Bryan, J.P. et al. (1995) J. Biol. Chem. 270:551.
Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics