Are the NS0 cells used to raise Recombinant Human Gas6 Protein, CF (Catalog # 885-GSB) cultured in the presence of Vitamin K?

Name: Recombinant Human Gas6 Protein, CF
Brand: R&D Systems
SKU: 885-GSB
Rating: 5 (6 reviews)

Yes, the NS0 cells are cultured in the presence of Vitamin K.

Recombinant Human Gas6 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 885-GSB

Citations (30)

(6)

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Key Product Details

Source:	NS0
Accession #:	NP_000811
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Gas6 Protein, CF

Product Specifications for Recombinant Human Gas6 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Gas6 protein Ala49-Ala678, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala49
Predicted Molecular Mass	70.5 kDa
SDS-PAGE	70-85 kDa, reducing conditions
Activity	Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED₅₀ for this effect is 25-150 ng/mL

Scientific Data Examples for Recombinant Human Gas6 Protein, CF

	Bioactivity of Recombinant Human Gas6 protein Recombinant Human Gas6 885-GSB down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED₅₀ for this effect is 25-150 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

885-GSB

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Citrate.
Reconstitution	Reconstitute at 500 μg/mL in sterile water. Reconstitution Buffer Available: Size / Price Qty Loading... RB07:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Gas6

Gas6 (Growth Arrest Specific 6) is a multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77%-79% aa sequence identity with mouse and rat Gas6, and 43% aa identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the
EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF-induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).

Item	Value
References	Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976. Stitt, T.N. et al. (1995) Cell 80:661. Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022. Goruppi, S. et al. (1997) FEBS Lett. 415:59. Sather, S. et al. (2007) Blood 109:1026. Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324. Shankar, S.L. et al. (2006) J. Neurosci. 26:5638. Yanagita, M. et al. (2002) J. Clin. Invest. 110:239. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733. Hall, M.O. et al. (2002) Exp. Eye Res. 75:391. Gallicchio, M. et al. (2005) Blood 105:1970. Caraux, A. et al. (2006) Nat. Immunol. 7:747. Wu, Y. et al. (2005) J. Cell Sci. 118:539. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
Long Name	Growth-arrest-specific Protein 6
Entrez Gene IDs	2621 (Human); 14456 (Mouse)
Alternate Names	AXLLG, AXLLGAXL stimulatory factor, AXSFAXL receptor tyrosine kinase ligand, DKFZp666G247, FLJ34709, GAS-6, Gas6, growth arrest-specific 6, growth arrest-specific protein 6

Citations for Recombinant Human Gas6 Protein, CF (30)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Gas6 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Iida et al. (2022), AXL regulates neuregulin1 expression leading to cetuximab resistance in head and neck cancer BMC Cancer, 22(1):447.
PMID: 35461210
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Puddu et al. (2022), High Glucose Impairs Expression and Activation of MerTK in ARPE-19 Cells International Journal of Molecular Sciences, 23(3).
PMID: 35163068
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

LW Sun et al. (2021), Corosolic Acid Attenuates the Invasiveness of Glioblastoma Cells by Promoting CHIP-Mediated AXL Degradation and Inhibiting GAS6/AXL/JAK Axis Cells, 10(11).
PMID: 34831142
Species: Human
Sample Types: Protein
Applications: ELISA (Capture)

H Chang et al. (2021), Anti-Axl monoclonal antibodies attenuate the migration of MDA-MB-231 breast cancer cells Oncology Letters, 22(5):749.
PMID: 34539853
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Wu et al. (2021), Quantitative Tyrosine Phosphoproteome Profiling of AXL Receptor Tyrosine Kinase Signaling Network Cancers, 13(16).
PMID: 34439388
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Standard

A Abu-Thurai et al. (2020), AXL confers cell migration and invasion by hijacking a PEAK1-regulated focal adhesion protein network Nat Commun, 11(1):3586.
PMID: 32681075
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

S Alivernini et al. (2020), Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis Nat. Med., 0(0).
PMID: 32601335
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

T Hara et al. (2020), Cabozantinib inhibits AXL- and MET-dependent cancer cell migration induced by growth-arrest-specific 6 and hepatocyte growth factor Biochem Biophys Rep, 21(0):100726.
PMID: 32055714
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Xue et al. (2020), CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK Arthritis Research Therapy, 2020;22(1):221.
PMID: 32958023
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

W Du et al. (2020), KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway Cell Death Differ, 0(0).
PMID: 33139930
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Pietrobono et al. (2020), ST3GAL1 is a target of the SOX2-GLI1 transcriptional complex and promotes melanoma metastasis through AXL Nat Commun, 11(1):5865.
PMID: 33203881
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Sinik et al. (2019), Inhibition of MERTK Promotes Suppression of Tumor Growth in BRAF Mutant and BRAF Wild-Type Melanoma Mol. Cancer Ther., 18(2):278-288.
PMID: 30482852
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KA Mills et al. (2019), p5RHH nanoparticle-mediated delivery of AXL siRNA inhibits metastasis of ovarian and uterine cancer cells in mouse xenografts Sci Rep, 9(1):4762.
PMID: 30886159
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

DP Strange et al. (2019), Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells MBio, 10(4).
PMID: 31311882
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Azad et al. (2019), A gain-of-functional screen identifies the Hippo pathway as a central mediator of receptor tyrosine kinases during tumorigenesis Oncogene, 0(0).
PMID: 31477837
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

A Tutusaus et al. (2019), A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET Cell Mol Gastroenterol Hepatol, 0(0).
PMID: 31689560
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

D De Silva et al. (2019), Interaction between androgen receptor and coregulator SLIRP is regulated by Ack1 tyrosine kinase and androgen Sci Rep, 9(1):18637.
PMID: 31819114
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

JP Hoj et al. (2019), A TAZ-AXL-ABL2 Feed-Forward Signaling Axis Promotes Lung Adenocarcinoma Brain Metastasis Cell Rep, 29(11):3421-3434.e8.
PMID: 31825826
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

F Collina et al. (2019), AXL Is a Novel Predictive Factor and Therapeutic Target for Radioactive Iodine Refractory Thyroid Cancer Cancers (Basel), 11(6).
PMID: 31181609
Sample Types: Complex Sample Type
Applications: Bioassay

K Fujita et al. (2018), Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology Nat Commun, 9(1):433.
PMID: 29382817
LC Cheng et al. (2018), AXL phosphorylates and up-regulates TNS2 and its implications in IRS-1-associated metabolism in cancer cells J. Biomed. Sci., 25(1):80.
PMID: 30419905
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Xu et al. (2017), BCL6 promotes glioma and serves as a therapeutic target Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28356518
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

ML Palisoul et al. (2017), Inhibition of the Receptor Tyrosine Kinase AXL Restores Paclitaxel Chemosensitivity in Uterine Serous Cancer Mol. Cancer Ther., 0(0).
PMID: 28904132
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay

SN Cross et al. (2017), Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation J. Immunol., 0(0).
PMID: 28916522
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Dantas-Bar et al. (2017), Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma Br. J. Cancer, 117(12):1787-1797.
PMID: 29024938
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zhi-Min Yuan (2016), AXL receptor signalling suppresses p53 in melanoma through stabilization of the MDMX-MDM2 complex J Mol Cell Biol, 0(0).
PMID: 27927748
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KE Happonen et al. (2016), The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles J. Biol. Chem., 291(20):10586-601.
PMID: 27006397
Applications: Western Blot

Wilhem Leconet (2016), Therapeutic activity of anti-AXL antibody against triple-negative breast cancer Patient Derived Xenografts and metastasis Clin. Cancer Res, 0(0).
PMID: 27923843
Applications: ELISA (Standard)

Kasikara C et al. (0), Phosphatidylserine Sensing by TAM Receptors Regulates AKT-Dependent Chemoresistance and PD-L1 Expression. Mol Cancer Re15(6):753-764.
PMID: 28184013
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

Y Duan et al. (0), Engineered AXL(-ECD)-Fc variants that abolish the AXL/Gas6 interaction suppress tumor cell migration. Oncol Let17(6):5784-5792.
PMID: 31186805

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Customer Reviews for Recombinant Human Gas6 Protein, CF (6)

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Recombinant Human Gas6 Protein, CF

Name: Anonymous

Application: SDS-PAGE Control

Reason for Rating: Confirmed purity via reduced PAGE.

Verified Customer | Posted 08/19/2022
Recombinant Human Gas6 Protein, CF

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 06/30/2021
Recombinant Human Gas6 Protein, CF

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 01/04/2019
Recombinant Human Gas6 Protein, CF

Name: Ruchi Gupta

Application: SDS-PAGE Control

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Application: Binding assay/Protein-protein interaction

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FAQs for Recombinant Human Gas6 Protein, CF

Are the NS0 cells used to raise Recombinant Human Gas6 Protein, CF (Catalog # 885-GSB) cultured in the presence of Vitamin K?

Yes, the NS0 cells are cultured in the presence of Vitamin K.

Recombinant Human Gas6 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Gas6 Protein, CF

Scientific Data Examples for Recombinant Human Gas6 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Gas6

Citations for Recombinant Human Gas6 Protein, CF (30)

Customer Reviews for Recombinant Human Gas6 Protein, CF (6)

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Recombinant Human Gas6 Protein, CF

Recombinant Human Gas6 Protein, CF

Recombinant Human Gas6 Protein, CF

Recombinant Human Gas6 Protein, CF

Recombinant Human Gas6 Protein, CF

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