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Recombinant Human Active ERK2 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1230-KS

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1230-KS-010

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human ERK2 protein

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N terminal GST tag

SDS-PAGE

68 kDa

Activity

The specific activity of ERK2 was determined to be 24 nmol/min/mg using a myelin basic protein (MBP) substrate and was equivalent to 639 nmol/min/mg as per radiometric assay.

Scientific Data Images for Recombinant Human Active ERK2 Protein, CF

Recombinant Human Active ERK2 Protein SDS-PAGE

Recombinant Human Active ERK2 Protein SDS-PAGE

The approximate molecular weight is 68 kDa and the purity is > 80%.

Formulation, Preparation and Storage

1230-KS
Formulation Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: ERK2

ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK2 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK2 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).

References

  1. Boulton, T.G. et al. (1991) Biochemistry 30(1):278.

Long Name

Extracellular Signal-regulated Kinase 2

Alternate Names

ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2

Entrez Gene IDs

5594 (Human); 26413 (Mouse); 116590 (Rat)

Gene Symbol

MAPK1

Additional ERK2 Products

Product Documents for Recombinant Human Active ERK2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active ERK2 Protein, CF

For research use only

Citations for Recombinant Human Active ERK2 Protein, CF (4)

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FAQs for Recombinant Human Active ERK2 Protein, CF

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  • Q: What is the activation protocol for this enzyme?

    A: This enzyme is supplied in its activated form, so no activation step is required.

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