Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF

Name: Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 838-4B
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 838-4B

Citations (5)

(1)

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Key Product Details

Source:	NS0
Accession #:	Q07011
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications for Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human 4-1BB/TNFRSF9/CD137 protein

Human 4-1BB (Leu24-Gln186) Accession # Q07011	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu24

Predicted Molecular Mass

44.8 kDa (monomer)

SDS-PAGE

50-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When recombinant human 4-1BB Fc Chimera is Immobilized at 10 ng/mL (100 µL/well), the concentration of recombinant human 4-1BB Ligand (Catalog # 2295-4L) that produces 50% optimal binding response is found to be approximately 0.5‑2.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

838-4B

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 4-1BB/TNFRSF9/CD137

4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells (1). Mature human 4-1BB consists of a 163 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine‑rich repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain (2, 3). Within the ECD, human 4-1BB shares 60% aa sequence identity with mouse and rat 4-1BB. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4⁺, CD8⁺, memory CD8⁺, NKT, and regulatory T cells (4-7) as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts (8-11). It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells (3, 8). This interaction co‑stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell (3-7). It can also enhance the activation-induced cell death of repetitively stimulated T cells (3). Mice lacking 4-1BB show augmented T cell activation, perhaps due to its absence on regulatory T cells (12). 4-1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8⁺ T cell proliferation (13). Reverse signaling through 4-1BB Ligand inhibits the development of dendritic cells, B cells, and osteoclasts (8, 11) but supports mature dendritic cell survival and co‑stimulates the proliferation and activation of mast cells (9, 10).
4‑1BB activation enhances CD8⁺ T cell and NK cell mediated anti-tumor immunity (14). It also contributes to the development of inflammation in high fat diet-induced metabolic syndrome (15). Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively (16, 17).

Item	Value
References	Wang, C. et al. (2009) Immunol. Rev. 229:192. Schwarz, H. et al. (1993) Gene 134:295. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219. Wen, T. et al. (2002) J. Immunol. 168:4897. Pulle, G. et al. (2006) J. Immunol. 176:2739. Zheng, G. et al. (2004) J. Immunol. 173:2428. Kim, D. et al. (2008) J. Immunol. 180:2062. Lee, S. et al. (2008) Nat. Immunol. 9:917. Choi, B.K. et al. (2009) J. Immunol. 182:4107. Nishimoto, H. et al. (2005) Blood 106:4241. Saito, K. et al. (2004) J. Biol. Chem. 279:13555. Lee, S. et al. (2005) J. Immunol. 174:6803. Ma, B.Y. et al. (2005) Blood 106:2002. Choi, B.K. et al. (2010) J. Immunol. 185:1404. Kim, C. et al. (2011) Diabetes 60:3159. Michel, J. et al. (1998) Eur. J. Immunol. 28:290. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
Entrez Gene IDs	3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)
Alternate Names	4-1BB, 4-1BB ligand receptor, 41BB, CD137, CD137 antigen, CD137MGC2172, CDw137, FLJ43501, ILA, ILAhomolog of mouse 4-1BB, T cell antigen ILA, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, induced by lymphocyte activation (ILA), interleukin-activated receptor, homolog of mouse Ly63, receptor protein 4-1BB, tumor necrosis factor receptor superfamily member 9, tumor necrosis factor receptor superfamily, member 9

Citations for Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Weigand et al. (2021), Soluble CD137 is a novel serum marker of liver cirrhosis in patients with hepatitis C and alcohol-associated disease etiology European Journal of Immunology, 0(0).
PMID: 34914098
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Transcriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype Sci Rep, 6(0):29712.
PMID: 27431276
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Moh M et al. (2013), Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling. FASEB J, 27(8):2957-66.
PMID: 23620528
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Harfuddin Z et al. (2013), CD137L-stimulated dendritic cells are more potent than conventional dendritic cells at eliciting cytotoxic T-cell responses. Oncoimmunology, 2(11):e26859.
PMID: 24482752
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Maniar A et al. (2010), Human gammadelta T lymphocytes induce robust NK cell-mediated antitumor cytotoxicity through CD137 engagement. Blood, 116(10):1726-33.
PMID: 20519625

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Customer Reviews for Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF (1)

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Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF

Name: Jenna Nguyen

Application: Apoptosis assay

Verified Customer | Posted 07/27/2018

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Product Documents for Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF

Product Datasheets

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COA

SDS

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Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: 4-1BB/TNFRSF9/CD137

Citations for Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF (5)

Customer Reviews for Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF (1)

Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF

Product Documents for Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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