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Recombinant Human 4-1BB/TNFRSF9 Fc Avi-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # AVI10536

Biotinylated
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AVI10536-050

Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human 4-1BB/TNFRSF9/CD137 protein
Human 4-1BB/TNFRSF9/CD137
(Leu24-Gln186)
Accession # Q07011.1
IEGRMD Human IgG1
(Pro100-Lys330)
Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu24

Predicted Molecular Mass

46 kDa

SDS-PAGE

56-66 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human 4-1BB Ligand/TNFSF9 His-tag (Catalog # 2295-4L) is immobilized at 0.01 µg/mL (100 µL/well), Biotinylated Recombinant Human 4-1BB/TNFRSF9/CD137 Fc Chimera Avi-tag (Catalog # AVI10536) binds with an ED50 of 0.125-1 ng/mL.

Scientific Data Images for Recombinant Human 4-1BB/TNFRSF9 Fc Avi-tag Protein, CF

Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Avi-tag Binding Activity.

When Recombinant Human 4-1BB Ligand/TNFSF9 His-tag (2295-4L) is immobilized at 0.01 µg/mL (100 µL/well), Biotinylated Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Avi-tag (Catalog # AVI10536) binds with an ED50 of 0.125-1 ng/mL.

Biotinylated Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Avi-tag SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Avi-tag Protein (Catalog # AVI10536) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-66 kDa and 110-132 kDa, respectively.

Formulation, Preparation and Storage

AVI10536
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB/TNFRSF9/CD137

4-1BB, also known as CD137 and TNFRSF9, is glycoprotein in the TNF receptor superfamily of immune costimulatory molecules. 4-1BB functions in the development and activation of multiple immune cell types (1). Mature human 4-1BB consists of an extracellular domain (ECD) with four TNFR cysteine‑rich repeats, a transmembrane segment and a cytoplasmic domain with four potential phosphorylation sites (2, 3). Within the ECD, human 4-1BB shares 60% amino acid sequence identity with mouse and rat 4-1BB. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells (4-7) as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts (8-11). It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells (3, 8). This interaction co-stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell (3-7). It can also enhance the activation-induced cell death of repetitively stimulated T cells (3). Mice lacking 4-1BB show augmented T cell activation, perhaps due to its absence on regulatory T cells (12). 4-1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8+ T cell proliferation (13). Reverse signaling through 4-1BB Ligand inhibits the development of dendritic cells, B cells, and osteoclasts (8, 11) but supports mature dendritic cell survival and co‑stimulates the proliferation and activation of mast cells (9, 10). 4‑1BB activation enhances CD8+ T cell and NK cell mediated anti-tumor immunity (14). It also contributes to the development of inflammation in high fat diet-induced metabolic syndrome (15). Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively (16, 17). Our Avi-tag Biotinylated 4‑1BB features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Wang, C. et al. (2009) Immunol. Rev. 229:192.
  2. Schwarz, H. et al. (1993) Gene 134:295.
  3. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  4. Wen, T. et al. (2002) J. Immunol. 168:4897.
  5. Pulle, G. et al. (2006) J. Immunol. 176:2739.
  6. Zheng, G. et al. (2004) J. Immunol. 173:2428.
  7. Kim, D. et al. (2008) J. Immunol. 180:2062.
  8. Lee, S. et al. (2008) Nat. Immunol. 9:917.
  9. Choi, B.K. et al. (2009) J. Immunol. 182:4107.
  10. Nishimoto, H. et al. (2005) Blood 106:4241.
  11. Saito, K. et al. (2004) J. Biol. Chem. 279:13555.
  12. Lee, S. et al. (2005) J. Immunol. 174:6803.
  13. Ma, B.Y. et al. (2005) Blood 106:2002.
  14. Choi, B.K. et al. (2010) J. Immunol. 185:1404.
  15. Kim, C. et al. (2011) Diabetes 60:3159.
  16. Michel, J. et al. (1998) Eur. J. Immunol. 28:290.
  17. Salih, H.R. et al. (2001) J. Immunol. 167:4059.

Alternate Names

41BB, CD137, ILA, TNFRSF9

Entrez Gene IDs

3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)

Gene Symbol

TNFRSF9

UniProt

Additional 4-1BB/TNFRSF9/CD137 Products

Product Documents for Recombinant Human 4-1BB/TNFRSF9 Fc Avi-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human 4-1BB/TNFRSF9 Fc Avi-tag Protein, CF

For research use only

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