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Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF

Catalog # 10482-R2 | R&D Systems, Inc. a Bio-Techne Brand
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10482-R2-100

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived IL-21R protein
Cynomolgus Monkey/Rhesus Macaque IL‑21R
(Cys20-Pro236)
Accession # XP_005591578.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys20

Predicted Molecular Mass

52 kDa

SDS-PAGE

60-80 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-21 Protein  (Catalog # 8879-IL) is immoblized at 2.5 µg/mL (100 µL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera (Catalog # 10482-R2) that produces a 50% optimal binding response is found to be 0.6-3.6 µg/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF

Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein Binding Activity

Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein Binding Activity

When Recombinant Human IL-21 Protein (8879-IL) is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera (10482-R2) that produces a 50% optimal binding response is found to be 0.6-3.6 μg/mL.
Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera Protein (Catalog # 10482-R2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa and 110-160 kDa, respectively.

Formulation, Preparation and Storage

10482-R2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-21R

Interleukin-21 Receptor (IL-21 R) is a type I transmembrane glycoprotein within the class I cytokine receptor family (1). IL-21 R associates with the common  gamma chain ( gammac) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 (2, 3). Based on its similarity with human IL-21 R, mature cynomolgus/rhesus IL-21 R is predicted to consist of a 213 amino acid (aa) extracellular domain (ECD), a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 253 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). Within the ECD, cynomolgus/rhesus IL-21 R shares 98% aa identity with human IL-21 R. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 4, 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (6). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses (7, 8). IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production (4, 9), control of viral infection and tumor growth by CD8+ T cells (10), development of regulatory T cells (11), IL-23 responsiveness of encephalitogenic Th17 cells (12), but suppresses the accumulation of IL-17 secreting gamma delta T cells in the airway (13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (14, 15).

References

  1. Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107.
  2. Asao, H. et al. (2001) J. Immunol. 167:1.
  3. Habib, T. et al. (2002) Biochemistry 41:8725.
  4. Parrish-Novak, et al. (2000) Nature 408:57.
  5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  6. Ozaki, K. et al. (2002) Science 298:1630.
  7. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
  8. King, I.L. et al. (2010) J. Immunol. 185:6138.
  9. Kasaian, M.T. et al. (2002) Immunity 16:559.
  10. Frohlich, A. et al. (2009 Science 324:1576.
  11. Tortola, L. et al. (2010) Blood 116:5200.
  12. Lee, Y. et al. (2015) J. Clin. Invest. 125:4011.
  13. Moser, E.K. et al. (2015) PLoS One 10:e0120169.
  14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
  15. Sarosiek, K.A. et al. (2010) Blood 115:570.

Long Name

Interleukin 21 Receptor

Alternate Names

CD360, IL-21 R, IL21R, NILR

Entrez Gene IDs

50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)

Gene Symbol

IL21R

UniProt

Product Documents for Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF

For research use only

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