Recombinant Cynomolgus PD-1 His-tag Protein, CF

Name: Recombinant Cynomolgus PD-1 His-tag Protein, CF
Brand: R&D Systems
SKU: 8509-PD
Price: 394 USD

Catalog # 8509-PD | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	NP_001271065.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus PD-1 His-tag Protein, CF

Product Specifications for Recombinant Cynomolgus PD-1 His-tag Protein, CF

Source	Human embryonic kidney cell, HEK293-derived cynomolgus monkey PD-1 protein Leu25-Gln167, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu25
Predicted Molecular Mass	17 kDa
SDS-PAGE	29-43 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human PD-L2/B7-DC Fc Chimera (Catalog # 1224-PL) is coated at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey PD-1, in the presence of Ms x Poly His, that produces 50% optimal binding response is typically 0.3-1.5 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8509-PD

Formulation	Lyophilized from a 0.2 μm filtered solution in sterile PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PD-1

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature Cynomolgus monkey PD-1 consists of a 148 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The Cynomolgus monkey PD-1 ECD shares 95% aa sequence identity with the human PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 and PD-L2 (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12, 13).

Item	Value
References	Ishida, Y. et al. (1992) EMBO J. 11:3887. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265. Watanabe, N et al. (2003) Nat. Immunol. 4:670. Zhang, X. et al. (2004) Immunity 20:337. Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483. Nishimura, H et al. (1996) Int. Immunol. 8:773. Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677. Butte, M.J. et al. (2007) Immunity 27:111. Okazaki, T. et al. (2013) Nat. Immunol. 14:1212. Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 12293. Nogrady, B. (2014) Nature 513:S10.
Long Name	Programmed Death-1
Entrez Gene IDs	5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names	CD279, CD279 antigen, PD-1, PD1, PD1hPD-l, PDCD1, Protein PD-1, SLEB2, hPD-1, programmed cell death 1, programmed cell death protein 1

Citations for Recombinant Cynomolgus PD-1 His-tag Protein, CF (1)

Showing 1 - 1 of 1 citation Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Cynomolgus PD-1 His-tag Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Issafras et al. (2021-12-31), Structural basis of HLX10 PD-1 receptor recognition, a promising anti-PD-1 antibody clinical candidate for cancer immunotherapy PLoS ONE, 2021-116(12):e0257972.
PMID: 34972111

There are no citations that match your criteria.

Showing 1 - 1 of 1 citation Showing All

Product Documents for Recombinant Cynomolgus PD-1 His-tag Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Cynomolgus PD-1 His-tag Protein, CF

Catalog # 8509-PD | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased