Source	Mouse myeloma cell line, NS0-derived cynomolgus monkey OX40/TNFRSF4 protein Leu29-Ala216, with an C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu29
Predicted Molecular Mass	21 kDa
SDS-PAGE	40-55 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey OX40/TNFRSF4 His tag (Catalog # 10137-OX) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) binds with an ED₅₀ of 0.25-1.5 ng/mL.

Recombinant Cynomolgus OX40/TNFRSF4 His-tag Protein, CF Scientific Data Examples

	Recombinant Cynomolgus OX40/TNFRSF4 His-tag Protein Binding Activity When Recombinant Cynomolgus Monkey OX40/TNFRSF4 His-tag (Catalog # 10137-OX) is immobilized at 0.25 µg/mL, 100 µL/well, Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) binds with an ED₅₀ of 0.25-1.5 ng/mL.
	Recombinant Cynomolgus OX40/TNFRSF4 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Cynomolgus Monkey OX40/TNFRSF4 His-tag (Catalog # 10137-OX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-55 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10137-OX

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OX40/TNFRSF4

OX40 (CD134; TNFRSF4) is a T cell co-stimulatory molecule of the TNF receptor superfamily that coordinates with other co-stimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response (1-3). Human OX40 is a 29 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 188 aa extracellular domain (ECD) that contains a cysteine-rich region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain (4). The ECD of cynomolgus OX40 shares 95% sequence identity with the ECD of human, 65% with mouse, and 64% with rat OX40. OX40 is up-regulated on CD4⁺ and CD8⁺ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7 (5, 6). OX40 Ligand is primarily expressed on antigen presenting cells (5). OX40 Ligand engagement of OX40 on activated CD4⁺ T cells results in increased T cell survival, proliferation, and cytokine production. It also inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs) and can promote the maintenance of and recall response in memory T cells (3, 7-10). OX40 is constitutively expressed on Tregs and enhances the sensitivity of Tregs to IL-2, thus promoting Treg proliferation. OX40 has also been shown to decrease the cells' immunosuppressive activity on effector T cells (11-14). OX40-OX40 Ligand signaling is involved in allergic airway inflammation, graft-versus-host disease and autoimmune disease (6, 15, 16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (17, 18).

Item	Value
References	Hori, T. (2006) Int. J. Hematol. 83:17. Latza, U. et al. (1994) Eur. J. Immunol. 24:677. Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315. al-Shamkhani, A. et al. (1996) Eur. J. Immunol. 26:1695. Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230. Gramaglia, I. et al. (1998) J. Immunol. 161:6510. Xiao, X. et al. (2008) J. Immunol. 181:3193. So, T. and M. Croft (2007) J. Immunol. 179:1427. Mousavi, S.F. et al. (2008) J. Immunol. 181:5990. Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907. Piconese, S. et al. (2010) Eur. J. Immunol. 40:2902. Griseri, T. et al. (2010) J. Exp. Med. 207:699. Xiao, X. et al. (2012) J. Immunol. 188:892. Vu, M.D. et al. (2007) Blood 110:2501. Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688.
Entrez Gene IDs	7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)
Alternate Names	ACT-135, ACT35 antigen, ACT35ATC35 antigen, CD134, CD134 antigen, Ly-70, OX40, OX40 cell surface antigen, OX40 homologue, OX40L receptor, OX40lymphoid activation antigene ACT35, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4, TXGP1L, TXGP1LOX40 antigen, Txgp1, tax-transcriptionally activated glycoprotein 1 receptor, tumor necrosis factor receptor superfamily member 4, tumor necrosis factor receptor superfamily, member 4

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Recombinant Cynomolgus OX40/TNFRSF4 His-tag Protein, CF

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