Recombinant Cynomolgus Monkey ErbB2/Her2 Fc Protein, CF

Name: Recombinant Cynomolgus Monkey ErbB2/Her2 Fc Protein, CF
Brand: R&D Systems
SKU: 10526-ER

Catalog # 10526-ER

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	CHO
Accession #:	XP_005584091.2
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus Monkey ErbB2/Her2 Fc Protein, CF

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey ErbB2/Her2 protein

Cynomolgus Monkey ErbB2/Her2 (Thr23-Thr652) Accession # XP_005584091.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Thr23

Predicted Molecular Mass

96 kDa

SDS-PAGE

115-140 kDa, under reducing conditions

Activity

Measured by its ability to block anti-ErbB2 mediated inhibition of SK‑BR‑3 human breast cancer cell proliferation. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875. The ED₅₀ for this effect is 0.2-1.2 μg/mL in the presence of 0.6 µg/mL Anti-Human ErbB2/Her2 Monoclonal Antibody (Trastuzumab, Catalog # MAB9589).

Recombinant Cynomolgus Monkey ErbB2/Her2 Fc Protein, CF Scientific Data Examples

	Recombinant Cynomolgus Monkey ErbB2/Her2 Fc Protein SDS-PAGE 2 μg/lane of Recombinant Cynomolgus Monkey ErbB2/Her2 Fc Protein (10526-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-140 kDa and 230-280 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10526-ER

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Mass (in vial)

Desired Reconstitution Concentration

Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I transmembrane glycoprotein member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factors. It is widely expressed in epithelial cells and has also been found to be over‑expressed in a large number of breast carcinomas. Among the ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Human ErbB2 consists of 1255 amino acids (aa) including a 22 aa signal sequence, a 630 aa extracellular domain (ECD), a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. Within the ECD, cynomolgus ErbB2 shares and 99% aa sequence identity with human ErbB2. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).

Item	Value
References	Coussens, L. et al. (1985) Science 230:1132. Yamamoto, T. et al. (1986) Nature 319:230. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067. Codony-Servat, J. et al. (1999) Cancer Res. 59:1196. Carraway, K.L. 3rd et al. (1994) J. Biol. Chem. 269:14303. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859. Schlessinger, J. (2000) Cell 103:211. Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153. Daly, R.J. (1999) Growth Factors 16:255.
Long Name	Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs	2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names	CD340, CD340 antigen, EC 2.7.10, EGFR2, ErbB2, HER-2, HER2, HER2EC 2.7.10.1, MLN 19, MLN19, Metastatic lymph node gene 19 protein, NEUHER-2/neu, NGL, NGLTKR1, Neu Oncogene, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2, c-erb B2/neu protein, herstatin, neuroblastoma/glioblastoma derived oncogene homolog, p185erbB2, receptor tyrosine-protein kinase erbB-2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

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