Product Specifications for Recombinant Cynomolgus Monkey Axl His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Axl protein Glu33-Trp450, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
57-77 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey Axl His-tag (Catalog # 10884-AX) is immobilized at 0.250 µg/mL (100 µL/well), Recombinant Human GAS6 (Catalog # 885-GSB) binds with an ED50 of 0.050-0.500 ng/mL.
Scientific Data Examples for Recombinant Cynomolgus Monkey Axl His-tag Protein, CF
Recombinant Cynomolgus Monkey Axl His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Cynomolgus Monkey Axl His-tag Protein (Catalog # 10884-AX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-77 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
Axl, also known as Ufo and Ark, is a widely expressed 140 kDa glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis (1). Mature cynomolgus monkey Axl consists of a 426 aa extracellular domain (ECD) that contains two Ig-like domains and two fibronectin type III domains, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain that includes the tyrosine kinase domain (2). Within the ECD, cynomolgus monkey Axl shares approximately 97% aa sequence identity with human Axl. Axl binds the vitamin K-dependent protein Gas6 which triggers tyrosine autophosphorylation of the Axl cytoplasmic domain (3). Activation of Axl induces a broad range of activities including platelet aggregation and thrombus formation (4), macrophage and dendritic cell phagocytosis of apoptotic cells (5), NK cell development from hematopoietic progenitor cells (6), and in vivo angiogenesis (7). Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness (7, 8). It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow (9). It also functions as a cellular entry receptor for Gas6-opsonized lentiviruses (10). A 70-80 kDa soluble portion of the Axl ECD can be shed by proteolytic cleavage, and this fragment retains the ability to bind Gas6 (11, 12).
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