Recombinant Cynomolgus Angiopoietin-like 3 Protein, CF

Name: Recombinant Cynomolgus Angiopoietin-like 3 Protein, CF
Brand: rnd
SKU: 10052-AN

Catalog # 10052-AN

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Key Product Details

Source:	T. ni (baculovirus)
Accession #:	XP_005543242
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus Angiopoietin-like 3 Protein, CF

Product Specifications

Source	Trichoplusia ni, T. ni (baculovirus)-derived cynomolgus monkey Angiopoietin-like Protein 3/ANGPTL3 protein Ser17-Glu460, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser17
Predicted Molecular Mass	53 kDa
SDS-PAGE	56-65 kDa, reducing conditions
Activity	Measured by its ability to inhibit lipoprotein lipase activity. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770. The IC₅₀ for this effect is 1.5-9 μg/mL.

Recombinant Cynomolgus Angiopoietin-like 3 Protein, CF Scientific Data Examples

	Recombinant Cynomolgus Angiopoietin-like 3 Protein Bioactivity Recombinant Cynomolgus Monkey Angiopoietin-like Protein 3/ANGPTL3 (Catalog # 10052-AN) dose dependently inhibits Recombinant Humam LPL (Catalog # 9888-LL) activity with an IC50 of 1.5-9 µg/mL.
	Recombinant Cynomolgus Angiopoietin-like 3 Protein SDS-PAGE 2 μg/lane of Recombinant Cynomolgus Monkey Angiopoietin‑like Protein 3/ANGPTL3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie Blue staining, showing bands at 56-65 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10052-AN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and CHAPS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 3/ANGPTL3

ANGPTL3 is a secreted glycoprotein that is structurally related to the Angiopoietins (1-3). Mature ANGPTL3 contains an N-terminal coiled‑coil domain and a C‑terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do proteolytically separated N- and C-terminal fragments containing the coiled‑coil domain and fibrinogen-like domains, respectively (6, 7).ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids (8, 9). This activity requires a putative heparin-binding motif which is N-terminal to the coiled-coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6-8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (10). ANGPTL3 expression in vivo is up-regulated by LXR agonists and down-regulated by insulin, leptin, and agonists of TR beta or PPAR beta (11-14). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice (7, 8, 12). ANGPTL3 does not bind Tie1 or Tie2, but its fibrinogen-like domain interacts with integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (15). ANGPTL3, secreted by fetal liver, also promotes the expansion of hematopoietic stem cells (16). Mature Cynomolgus Monkey ANGPTL3 shares 97%, 77%, and 78% amino acid sequence identity with human, mouse, and rat ANGPTL3, respectively.

Item	Value
References	Li, C. (2006) Curr. Opin. Lipidol. 17:152. Oike, Y. et al. (2004) Int. J. Hematol. 80:21. Kersten, S. (2005) Biochem. Soc. Trans. 33:1059. Conklin, D. et al. (1999) Genomics 62:477. Ge, H. et al. (2005) J. Lipid Res. 46:1484. Ono, M. et al. (2003) J. Biol. Chem. 278:41804. Koishi, R. et al. (2002) Nat. Genet. 30:151. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742. Shimamura, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:366. Koster, A. et al. (2005) Endocrinology 146:4943. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553. Matsusue, K. et al. (2006) Mol. Cell Endocrinol. 256:23. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281. Zhang, C.C. et al. (2006) Nat. Med. 12:240.
Entrez Gene IDs	27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names	AGNPT5, ANGPT5angiopoietin-5, ANGPTL3, Ang-5, Angiopoietin-5, Angiopoietin-like Protein 3, FHBL2, angiopoietin 5, angiopoietin-like 3, angiopoietin-related protein 3

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