Recombinant Cotton Rat TNF-alpha Protein

Name: Recombinant Cotton Rat TNF-alpha Protein
Brand: rnd
SKU: 1011-CR

Catalog # 1011-CR

Citations (1)

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Key Product Details

Source:	E. coli
Accession #:	AAL18818
Applications:	Bioactivity

View Full Technical Details for Recombinant Cotton Rat TNF-alpha Protein

Product Specifications

Source	E. coli-derived cotton rat TNF-alpha protein Leu1-Leu156, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	17.5 kDa
Activity	Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED₅₀ for this effect is 0.03-0.12 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1011-CR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄, NaCl and EDTA with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1011-CR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄, NaCl and EDTA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). The 156 amino acid (aa) cotton rat TNF-alpha is homologous to a portion of the extracellular domain (ECD) of TNF-alpha from other species (3). It shares 72%-87% with the ECD of bovine, canine, equine, feline, human, mouse, porcine, rat, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).

Item	Value
References	Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335. Blanco, J.C. et al. (2004) J. Interferon Cytokine Res. 24:21. Tang, P. et al. (1996) Biochemistry 35:8216. Perez, C. et al. (1990) Cell 63:251. Black, R.A. et al. (1997) Nature 385:729. Moss, M.L. et al. (1997) Nature 385:733. Gearing, A.J.H. et al. (1994) Nature 370:555. Schall, T.J. et al. (1990) Cell 61:361. Loetscher, H. et al. (1990) Cell 61:351. Dembic, Z. et al. (1990) Cytokine 2:231. Smith, C.A. et al. (1990) Science 248:1019. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.
Long Name	Tumor Necrosis Factor alpha
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names	APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-A, TNF-alpha, TNF-alphacachectin, TNFA, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, TNFalpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor-alpha

Citations (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Cotton Rat TNF-alpha Protein include:

Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

X Gao et al. (2018), Ubiquitin Carboxyl Terminal Hydrolase L1 Attenuates TNF-?-Mediated Vascular Smooth Muscle Cell Migration Through Suppression of NF-?B Activation Int Heart J, 0(0):.
PMID: 30305579

Recombinant Cotton Rat TNF-alpha Protein

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Key Product Details

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Product Specifications

Formulation, Preparation and Storage

Carrier Free

1011-CR

Carrier

1011-CR/CF

Carrier Free

Reconstitution Calculator

Background: TNF-alpha

Citations (1)

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