Recombinant Canine PD-L1/B7-H1 Fc Chimera Protein, CF

Name: Recombinant Canine PD-L1/B7-H1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10746-B7

Catalog # 10746-B7

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	HEK293
Accession #:	NP_001278901.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Canine PD-L1/B7-H1 Fc Chimera Protein, CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived canine PD-L1/B7-H1 protein

Canine PD-L1/B7-H1 (Phe19-Arg236) Accession # NP_001278901.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe19

Predicted Molecular Mass

51 kDa

SDS-PAGE

60-80 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Canine PD-1 Fc Chimera (Catalog # 10553-PD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Canine PD-L1/B7-H1 Fc Chimera (Catalog # 10746-B7) binds with an ED₅₀ of 0.30-4.50 µg/mL.

Recombinant Canine PD-L1/B7-H1 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Canine PD-L1/B7-H1 Fc Chimera Protein Binding Activity. When Recombinant Canine PD-1 Fc Chimera (10553-PD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Canine PD-L1/B7-H1 Fc Chimera (Catalog # 10746-B7) binds with an ED₅₀ of 0.30-4.50 µg/mL.
	Recombinant Canine PD-L1/B7-H1 Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Canine PD-L1/B7-H1 Fc Chimera (Catalog # 10746-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa and 120-160 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10746-B7

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PD-L1/B7-H1

Programmed Death Ligand 1 (PD-L1), also known as B7-H1 and CD274, is a transmembrane glycoprotein in the B7 family of immune regulatory molecules. The B7 family is comprised of at least 10, structurally related members, and plays a central role in the regulation of T cell response by eliciting both positive co-stimulatory and negative inhibitory signals (1). Manipulation of B7 mediated signaling has shown potential in the treatment of autoimmunity, inflammatory diseases and cancer (1, 2). Mature PD-L1 consists of 1 IgV-like and 1 IgC-like region in the extracellular domain (ECD), a transmembrane region and a short intracellular domain. The ECD of canine PD-L1 shares 80% and 70% amino acid sequence identity with human and mouse PD-L1, respectively. In humans, alternative splicing can generate additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (3). PD-L1 binds to T cell B7-1/CD80 and PD-1 (4). PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, endothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma (4). It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells (5). It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development (5, 6). PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells (5-7). In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (8).

Item	Value
References	Greenwald, R.J. et al. (2005) Annual Review of Immunology 23:515. Collins, Mary et al. (2005) Genome biology 6:223. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915. Daassi, D. et al. (2020) Nat Rev Immunol. 20:209. Ray, A. et al. (2015) Leukemia. 29:1441. Zhao, Y. et al. (2018) Oncotarget. 9:14803. Herold, M. et al. (2015) J. Immunol. 195:3584. Jiang, Y. et al. (2020) Canc lett. 468:72.
Long Name	Programmed Death Ligand 1
Entrez Gene IDs	29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names	Avelumab, B7-H, B7-H1, B7H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PD-L1, PD-L1B7 homolog 1, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PDL1PDCD1 ligand 1, Programmed death ligand 1, programmed cell death 1 ligand 1

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