Janelia Fluor® 635, BromoCatch™ Ligand
Tocris Bioscience, part of Bio-Techne | Catalog # 8937
Key Product Details
| Description: | Fluorogenic Janelia Fluor® 635 conjugated covalent ligand for BromoCatch™, enabling no-wash live-cell imaging |
| Chemical Name: | 4-(((R)-14-((S)-4-(4-Acrylamidophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-13-oxo-3,6,9-trioxa-12-azahexadecyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 1152.4 |
Biological Activity
Janelia Fluor® 635, BromoCatch™ Ligand is a fluorogenic BromoCatch™ ligand functionalized with Janelia Fluor® 635, designed for real-time, no-wash imaging of BromoCatch-tagged proteins in live cells. Upon covalent binding to the BromoCatch™ tag, this probe undergoes a fluorescence switch-on due to the environmental sensitivity of the Janelia Fluor® 635 dye, delivering bright, red-shifted signal with minimal background fluorescence. Janelia Fluor® 635, BromoCatch™ Ligand enables nuclear-targeted labeling of fusion constructs such as H2B-BromoCatch, and shows no off-target activity in wild-type or untransfected cells. Compatible with super-resolution imaging and suitable for dynamic cellular studies, Janelia Fluor® 635, BromoCatch™ Ligand offers a powerful tool for live-cell tracking of BromoCatch-tagged proteins with excellent photostability and imaging contrast.
Please refer to this protocol for further information on how to use this product.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
BromoCatch is a trademark of Bio-Techne Corp.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus and the University of Dundee
Optical Data for Janelia Fluor® 635, BromoCatch™ Ligand
| λabs | 646 nm |
|---|---|
| λem | 663 nm |
| Cell Permeable | Yes |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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