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Mouse M-CSF Quantikine ELISA Kit

Bio-Techne includes R&D Systems | Catalog # MMC00B

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MMC00B

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.486 pg/mL

Assay Range

6.25-400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Product Summary for Mouse M-CSF Quantikine ELISA Kit

The Quantikine™ Mouse M-CSF Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse M-CSF levels in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant mouse M-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse M-CSF. Results obtained using natural mouse M-CSF showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards.  These results indicate that this kit can be used to determine relative mass values for natural mouse M-CSF.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Mouse

Specificity

Natural and recombinant mouse M-CSF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 218 213 57.8 23.0 224 11.2
Standard Deviation 1.05 9.97 1.72 1.61 14.8 0.765
CV% 4.8 4.7 3.0 7.0 6.6 6.8

Recovery for Mouse M-CSF Quantikine ELISA Kit

The recovery of mouse M-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 97-108
EDTA Plasma (n=4) 97 92-105
Heparin Plasma (n=4) 99 93-110
Serum (n=4) 94 88-98

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse M-CSF in each matrix were diluted with calibrator diluent and assayed.

Scientific Data Images for Mouse M-CSF Quantikine ELISA Kit

Mouse M-CSF Quantikine Spiked Recovery Competitor Comparison

M-CSF is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Plasma recovery is 103% compared to 84% for the top competitor. Cell culture supernates recovery is 100% compared to 75% for the top competitor.

Mouse M-CSF ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Gene Symbol

CSF1

Additional M-CSF Products

Product Documents for Mouse M-CSF Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse M-CSF Quantikine ELISA Kit

For research use only

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