Mouse M-CSF Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MMC00B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.486 pg/mL
Assay Range
6.25-400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse M-CSF Quantikine ELISA Kit
The Quantikine™ Mouse M-CSF Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse M-CSF levels in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant mouse M-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse M-CSF. Results obtained using natural mouse M-CSF showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse M-CSF.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse M-CSF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 218 | 213 | 57.8 | 23.0 | 224 | 11.2 |
| Standard Deviation | 1.05 | 9.97 | 1.72 | 1.61 | 14.8 | 0.765 |
| CV% | 4.8 | 4.7 | 3.0 | 7.0 | 6.6 | 6.8 |
Recovery for Mouse M-CSF Quantikine ELISA Kit
The recovery of mouse M-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 97-108 |
| EDTA Plasma (n=4) | 97 | 92-105 |
| Heparin Plasma (n=4) | 99 | 93-110 |
| Serum (n=4) | 94 | 88-98 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse M-CSF in each matrix were diluted with calibrator diluent and assayed.
Scientific Data Images for Mouse M-CSF Quantikine ELISA Kit
Mouse M-CSF Quantikine Spiked Recovery Competitor Comparison
M-CSF is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Plasma recovery is 103% compared to 84% for the top competitor. Cell culture supernates recovery is 100% compared to 75% for the top competitor.
Mouse M-CSF ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: M-CSF
M-CSF (CSF-1) is the primary regulator of macrophage survival, proliferation and differentiation. It is essential for macrophage-related functions such as bone resorption, vascular development, and innate immunity. It signals through M-CSF R on monocytes, tissue macrophages, and osteoblasts. Both M-CSF and its receptor can be expressed by tumor cells, allowing M-CSF to act as an autocrine growth factor for cancer cells. Full length M-CSF is expressed as a transmembrane dimer, but various additional forms can be generated by alternative splicing, differential glycosylation, chondroitin sulfate proteoglycan modification, and proteolytic shedding.
Long Name
Macrophage Colony Stimulating Factor
Alternate Names
CSF-1, CSF1, Lanimostim, MCSF
Gene Symbol
CSF1
Additional M-CSF Products
Product Documents for Mouse M-CSF Quantikine ELISA Kit
Product Specific Notices for Mouse M-CSF Quantikine ELISA Kit
For research use only
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