Human VE-Cadherin DuoSet ELISA

Name: Human VE-Cadherin DuoSet ELISA
Brand: R&D Systems
SKU: DY938-05

Catalog # DY938-05 | R&D Systems, Inc. a Bio-Techne Brand

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	0.312 - 20 ng/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human VE-Cadherin DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

View Full Technical Details for Human VE-Cadherin DuoSet ELISA

Technical Data
Citations / Reviews

Summary for Human VE-Cadherin DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human VE-Cadherin. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human VE-Cadherin DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human VE-Cadherin DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Human VE-Cadherin DuoSet ELISA

	Human VE-Cadherin ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VE-Cadherin

The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2⁺-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.

Item	Value
Long Name	Vascular Endothelium Cadherin
Entrez Gene IDs	1003 (Human); 12562 (Mouse)
Alternate Names	7B4, 7B4 antigen, CD144, CD144 antigen, CDH5, Cadherin-5, FLJ17376, VE-Cadherin, VECadherin, Vascular endothelial cadherin, cadherin 5, type 2 (vascular endothelium), cadherin 5, type 2, VE-cadherin (vascular epithelium), endothelial-specific cadherin

Citations for Human VE-Cadherin DuoSet ELISA

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Human VE-Cadherin DuoSet ELISA

Catalog # DY938-05 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for Human VE-Cadherin DuoSet ELISA

Product Specifications for Human VE-Cadherin DuoSet ELISA

Kit Contents for Human VE-Cadherin DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Human VE-Cadherin DuoSet ELISA

Preparation and Storage

Background: VE-Cadherin

Citations for Human VE-Cadherin DuoSet ELISA

Product Documents for Human VE-Cadherin DuoSet ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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