Human Total IL-18/IL-1F4 Quantikine ELISA Kit
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Length: | 4.0 hours |
Sample Type & Volume Required Per Well: | Cell Culture Supernates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL), Saliva (25 uL), Urine (50 uL) |
Sample Types: | Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine |
Sensitivity: | 5.15 pg/mL |
Assay Range: | 15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine) |
Human Total IL-18/IL-1F4 Quantikine ELISA Kit Summary: | The Quantikine® Human Total IL-18/IL-1F4 Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IL-18 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IL-18 and has been shown to accurately quantitate the recombinant factor free and in complex with IL-18 BPa. Results obtained using natural human IL-18 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18. |
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Citations for Human Total IL-18/IL-1F4 Quantikine ELISA Kit (16)
Citations are publications that use Bio-Techne products. Selected citations for Human Total IL-18/IL-1F4 Quantikine ELISA Kit include:
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Species: Human
Sample Types: Serum
DB Rosen et al. (2022), Cytokine response over the course of COVID-19 infection in pregnant women Cytokine, 154(0):155894.
PMID: 35490452 -
Species: Human
Sample Types: Plasma
F Piancone et al. (2022), Modulation of Neuroendocrine and Immunological Biomarkers Following Rehabilitation in Sarcopenic Patients Cells, 11(16).
PMID: 36010554 -
Species: Human
Sample Types: Cell Culture Supernates
F La Rosa et al. (2022), Modulation of MAPK- and PI3/AKT-Dependent Autophagy Signaling by Stavudine (D4T) in PBMC of Alzheimer's Disease Patients Cells, 11(14).
PMID: 35883623 -
Species: Human
Sample Types: Cell Culture Supernates
MC Chiang et al. (2022), Resveratrol Mitigates Oxygen and Glucose Deprivation-Induced Inflammation, NLRP3 Inflammasome, and Oxidative Stress in 3D Neuronal Culture International Journal of Molecular Sciences, 23(19).
PMID: 36232980 -
Species: Human
Sample Types: Serum
P Szczepanik et al. (2022), Serum Levels of Selected IL-1 Family Cytokines in Patients with Morphea Journal of Clinical Medicine, 11(21).
PMID: 36362603 -
Species: Human
Sample Types: Cell Culture Supernates
ML Matias et al. (2021), Progesterone and vitamin D downregulate the activation of the NLRP1/NLRP3 inflammasomes and TLR4-MyD88-NF-&kappaB pathway in monocytes from pregnant women with preeclampsia Journal of reproductive immunology, 144(0):103286.
PMID: 33578174 -
Species: Human
Sample Types: Plasma
F La Rosa et al. (2021), Deregulation of IL-37 and its miRNAs modulators in sarcopenic patients after rehabilitation Journal of Translational Medicine, 19(1):172.
PMID: 33902634 -
Species: Human
Sample Types: Serum
FS Thaler et al. (2020), Possible link of genetic variants to autoimmunity in GAD-antibody-associated neurological disorders J. Neurol. Sci., 413(0):116860.
PMID: 32388243 -
Species: Human
Sample Types: Serum
SR Kim et al. (2020), SGLT2 inhibition modulates NLRP3 inflammasome activity via ketones and insulin in diabetes with cardiovascular disease Nat Commun, 11(1):2127.
PMID: 32358544 -
Species: Human
Sample Types: Plasma
DM de Graaf et al. (2020), Reduced concentrations of the B cell cytokine Interleukin 38 are associated with cardiovascular disease risk in overweight subjects Eur J Immunol, 0(0).
PMID: 33125159 -
Species: Mouse
Sample Types: Cell Culture Supernates
H Tsugawa et al. (2020), Short-chain fatty acids bind to apoptosis-associated speck-like protein to activate inflammasome complex to prevent Salmonella infection PLoS Biol, 18(9):e3000813.
PMID: 32991574 -
Species: Human
Sample Types: Semen
L Qian (2018), Decreased interleukin-11 levels in the semen of infertile males Cytokine, 108(0):57-59.
PMID: 29573586 -
Species: Human
Sample Types: Cell Culture Supernates
L Janks et al. (2018), A central role for P2X7 receptors in human microglia J Neuroinflammation, 15(1):325.
PMID: 30463629 -
Species: Human
Sample Types: Serum
S Salimi et al. (2018), Inflammation and Trajectory of Renal Function in Community-Dwelling Older Adults J Am Geriatr Soc, 0(0).
PMID: 29360163 -
Species: Human
Sample Types: Plasma
YK Yong et al. (2017), Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease J Transl Med, 15(1):121.
PMID: 28569153 -
Species: Human
Sample Types: Urine
Tominaga T et al. (0), The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males. Antioxidants (Basel10(6).
PMID: 34071378
There are no citations that match your criteria.
Customer Reviews for Human Total IL-18/IL-1F4 Quantikine ELISA Kit (3)
Customer Images
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Sample Tested: SerumVerified Customer | Posted 01/27/2021Curve developed beautifully, kit is easy to use.
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Sample Tested: SerumVerified Customer | Posted 01/27/2021Picture of the plate before adding the stop solution.Standard curve in duplicate in columns 1 and 2.Assay ran in duplicate.Clear, precise and easy to follow directions.
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Sample Tested: Serum and PlasmaVerified Customer | Posted 01/25/2020
There are no reviews that match your criteria.
FAQs for Human Total IL-18/IL-1F4 Quantikine ELISA Kit
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Does the Human Total IL-18/IL-1F4 Quantikine ELISA Kit recognize both free and bound IL-18?
The Human Total IL-18/IL-1F4 Quantikine ELISA Kit detects free and complexed Human IL-18 in validated sample types.
Product Documents for Human Total IL-18/IL-1F4 Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.