Human PlGF Quantikine HS ELISA Kit

Name: Human PlGF Quantikine HS ELISA Kit
Brand: R&D Systems
SKU: HSPG00

Catalog # HSPG00 | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

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Key Product Details

Assay Length:	3.5 hours
Sample Type & Volume Required Per Well:	Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity:	0.326 pg/mL
Assay Range:	3.13 - 200 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Human PlGF Quantikine HS ELISA Kit Summary:	The Quantikine HS Human PlGF Immunoassay is a 3.5 hour solid phase ELISA designed to measure PlGF levels in serum and plasma. It contains E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF.

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Technical Data
Citations (4) / Reviews

Product Specifications for Human PlGF Quantikine HS ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human PlGF.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	27.2	57.4	108	27.3	57.7	108
Standard Deviation	0.72	1.87	2.84	1.65	3.88	7.37
CV%	2.6	3.3	2.6	6	6.7	6.8

Recovery

The recovery of PlGF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	98	85-115
Heparin Plasma (n=4)	90	86-97
Serum (n=4)	92	86-97

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of PlGF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human PlGF Quantikine HS ELISA Kit

	Human PlGF ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PlGF

PlGF (placenta growth factor) is a homodimeric growth factor that competes with VEGF for binding to VEGF R1/Flt-1. It therefore increases the availability of VEGF to bind to VEGF R2/KDR/Flk-1 and trigger angiogenesis. It can form heterodimers with some forms of VEGF and decrease the angiogenic effect of VEGF on VEGF R2. Circulating PlGF levels increase during pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia. PlGF induces monocyte activation and migration as well as production of inflammatory cytokines and VEGF. These activities facilitate wound, bone fracture, and cardiac repair, but also contribute to inflammation in active sickle cell disease and atherosclerosis. PlGF can also inhibit TIMP3 expression in the spleen, leading to immune triggering of hypertension.

Item	Value
Long Name	Placenta Growth Factor
Entrez Gene IDs	5228 (Human); 18654 (Mouse)
Alternate Names	D12S1900, PGF, PGFL, PLGFplacental growth factor-like, PlGF, PlGF-2, SHGC-10760, placenta growth factor, placental growth factor, placental growth factor, vascular endothelial growth factor-related protein

Citations for Human PlGF Quantikine HS ELISA Kit (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human PlGF Quantikine HS ELISA Kit include:

Species: Human
Sample Types: Serum

AN Burska et al. (2020-02-11), Dynamics of Early Signalling Events during Fracture Healing and Potential Serum Biomarkers of Fracture Non-Union in Humans J Clin Med, 2020-09(2).
PMID: 32054088
Species: Human
Sample Types: Serum

G Merckx et al. (2020-01-28), Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles Cells, 2020-09(2).
PMID: 32012900
Species: Mouse
Sample Types: Tissue Extracts

M Perrotta et al. (2018-03-01), Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation Cardiovasc. Res., 2018-0114(3):456-467.
PMID: 29324984
Species: Human
Sample Types: Plasma

JM Gu et al. (2018-01-11), Blockade of placental growth factor reduces vaso-occlusive complications in murine models of sickle cell disease Exp. Hematol., 2018-00(0).
PMID: 29337222

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