Human Peroxiredoxin 2 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY3489-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
0.781-50 ng/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Peroxiredoxin 2 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Peroxiredoxin 2 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Peroxiredoxin 2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Peroxiredoxin 2 DuoSet ELISA
Human Peroxiredoxin 2 ELISA Standard Curve
Kit Contents for Human Peroxiredoxin 2 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Peroxiredoxin 2
Peroxiredoxins make up a family of highly conserved peroxidases well known for their anti-oxidant properties. In this capacity they may be involved in the protection of cells from oxidative stress. They have roles as modulators of signal transduction, transcription, and apoptosis. To date, 6 family members have been described in mammals (peroxiredoxin 1 through 6). Peroxiredoxins are further subclassified based on the position of cysteine residues that are involved in catalysis (1-Cys, 2-Cys, and atypical 2-Cys).
Alternate Names
NKEF-B, PRDX2, PRX2, TDPX1, TSA
Gene Symbol
PRDX2
Additional Peroxiredoxin 2 Products
Product Documents for Human Peroxiredoxin 2 DuoSet ELISA
Product Specific Notices for Human Peroxiredoxin 2 DuoSet ELISA
For research use only
Loading...
Loading...
Loading...