Human PD-1 QuicKit ELISA

Name: Human PD-1 QuicKit ELISA
Brand: rnd
SKU: QK1086

Catalog # QK1086

Product Datasheet / COA / SDS

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Key Product Details

Assay Length:	80 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sample Types:	Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	9.86 pg/mL
Assay Range:	25 - 1,600 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Human PD-1 QuicKit ELISA Summary:	Natural and recombinant human PD-1.

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Product Specifications

Measurement	Quantitative ELISA
Conjugate	HRP
Reactivity	Human
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	20	20
Mean (pg/mL)	143	871	157	990
Standard Deviation	6.17	19.4	15	57.2
CV%	4.3	2.2	9.6	5.8

Recovery

The recovery of human PD-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	114	91-131
Cell Lysis Buffer (n=1)	117	94-133
EDTA Plasma (n=2)	104	91-122
Heparin Plasma (n=2)	85	71-103
Serum (n=2)	75	67-82

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human PD-1 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human PD-1 QuicKit ELISA Scientific Data Examples

	Human PD-1 ELISA Standard Curve
	Human PD-1 QuicKit Natural Linearity Competitor Comparison Samples containing and/or spiked with high concentrations of PD-1 in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 99%-109% compared to 105%-135% for the top competitor.

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PD-1

PD-1 (Programmed Death-1 receptor) is a key regulator of the threshold of immune response and peripheral immune tolerance. It is expressed on activated T cells, B cells, monocytes, and dendritic cells and binds to PD-L1 or PD-L2. PD-1 ligation induces co-inhibitory signals in T cells promoting their apoptosis, anergy, and functional exhaustion. Blockade of the PD-1: PD-L1 interaction using anti-PD-1 antibodies enhances understanding of anti-tumor immunity and advances the potential for cancer immunotherapy.

Item	Value
Long Name	Programmed Death-1
Entrez Gene IDs	5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names	CD279, CD279 antigen, PD-1, PD1, PD1hPD-l, PDCD1, Protein PD-1, SLEB2, hPD-1, programmed cell death 1, programmed cell death protein 1

Citations for Human PD-1 QuicKit ELISA

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