Human LAIR1 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY2664-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
39.1-2500 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human LAIR1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human LAIR1 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human LAIR1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as cell lysates, serum, and plasma should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human LAIR1 DuoSet ELISA
Human LAIR1 Standard Curve
Kit Contents for Human LAIR1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer:(Catalog #WA126), or 0.05% Tween®20 in PBS, pH 7.2-7.4
Reagent Diluent:(Catalog #DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution:1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog #DY999)
Stop Solution:2 N H2SO4(Catalog #DY994)
Microplates: R&D Systems (Catalog #DY990)
Plate Sealers:ELISA Plate Sealers (Catalog #DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LAIR1
LAIR1 (leukocyte-associated Ig-like receptor-1; CD305) is a transmembrane collagen-binding protein that is expressed in a differentiation- and activation-dependent manner on T, B, NK and dendritic cells, monocytes, CD34+ hematopoietic progenitors, most thymocytes, and select granulocyte populations. LAIR1 mediated adhesion to collagens in the extracellular matrix inhibits B and T cell receptor-mediated activation, NK and T cell-mediated cytotoxicity, and basophil degranulation. LAIR1 is reduced or absent on human B cell chronic lymphocytic leukemia (CLL) and some B and dendritic cells in systemic lupus erythematosus (SLE). A soluble form of LAIR1 found in plasma and urine also binds collagen.
Long Name
Leukocyte-associated Ig-like Receptor 1
Alternate Names
CD305, HLAIR1, LAIR-1
Gene Symbol
LAIR1
Additional LAIR1 Products
Product Documents for Human LAIR1 DuoSet ELISA
Product Specific Notices for Human LAIR1 DuoSet ELISA
For research use only
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