Human Integrin alpha 4 beta 1 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY5668-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
125-8000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Integrin alpha 4 beta 1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Integrin alpha 4 beta 1 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Integrin α4β1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Integrin alpha 4 beta 1 DuoSet ELISA
Human Integrin alphaa4 beta1 Standard Curve
Kit Contents for Human Integrin alpha 4 beta 1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer:(Catalog #WA126), or 0.05% Tween®20 in PBS, pH 7.2-7.4
Reagent Diluent:(Catalog #DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution:1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog #DY999)
Stop Solution:2 N H2SO4(Catalog #DY994)
Microplates: R&D Systems (Catalog #DY990)
Plate Sealers:ELISA Plate Sealers (Catalog #DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Integrin alpha 4 beta 1
Integrin alpha 4 beta 1, also known as VLA-4, is an Integrin family adhesion receptor that shares the beta 1 subunit with eleven other family members and the alpha 4 subunit with Integrin alpha 4 beta 7. The noncovalent heterodimer of alpha 4/CD49d and beta 1/CD29 subunits mediates cell adhesion to VCAM-1/CD106 on other cells and the CS-1 fragment of Fibronectin in the extracellular matrix. Leukocytes (except for neutrophils), erythroid precursors, and some non-hematopoietic cells such as epicardial, endothelial and smooth muscle precursors, Schwann cells, and chorionic cells express a4b1. In the adult, a4b1 primarily regulates immune cell migration. Circulating leukocyte a4b1 is rapidly activated by inflamed endothelial cells that present VCAM-1 and chemokines such as CXCL12/SDF-1. This activation facilitates rolling, firm adhesion, and extravasation. During immune cell activation, a4b1 can function as a costimulatory molecule.
Additional Integrin alpha 4 beta 1 Products
Product Documents for Human Integrin alpha 4 beta 1 DuoSet ELISA
Product Specific Notices for Human Integrin alpha 4 beta 1 DuoSet ELISA
For research use only
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