Human CD47 DuoSet ELISA

Name: Human CD47 DuoSet ELISA
Brand: rnd
SKU: DY4670-05
Rating: 4 (1 reviews)

Catalog # DY4670-05

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	125 - 8,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human CD47 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Human CD47 DuoSet ELISA Summary

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CD47. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Human CD47 DuoSet ELISA Kit Contents

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Human CD47 DuoSet ELISA Scientific Data Examples

	Human CD47 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD47

CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a ubiquitous transmembrane protein that binds to SIRP family members on macrophages, neutrophils, and T cells. These interactions prevent macrophage-mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium. CD47 associates in cis with Fas on T cells and enhances Fas-mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses. CD47 also associates in cis with several Integrins?which can positively or negatively modulate Integrin-mediated function. CD47 binding by Thrombospondin-1 inhibits the angiogenic and vasorelaxant effects of nitric oxide. On dendritic cells and myeloma cells, CD47 ligation by TSP-1 induces giant cell formation and osteoclast differentiation.

Item	Value
Entrez Gene IDs	961 (Human); 16423 (Mouse); 397042 (Porcine); 478552 (Canine); 102139846 (Cynomolgus Monkey)
Alternate Names	Antigenic surface determinant protein OA3, CD47, CD47 antigen, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, CD47 molecule, IAP, IAPintegrin associated protein, Integrin-associated protein, MER6, MER6integrin-associated signal transducer, OA3, Protein MER6, Rh-related antigen, antigen identified by monoclonal 1D8, leukocyte surface antigen CD47

Citations (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human CD47 DuoSet ELISA include:

Species: Human
Sample Types: Cell Lysates

(2016), A rapid, automated surface protein profiling of single circulating exosomes in human blood Sci Rep, 6(0):36502.
PMID: 27819324

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Human CD47 DuoSet ELISA

Name: Anonymous

Sample Tested: Serum and Plasma

Verified Customer | Posted 04/18/2021

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Human CD47 DuoSet ELISA

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Key Product Details

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Human CD47 DuoSet ELISA Summary

Product Specifications

Human CD47 DuoSet ELISA Kit Contents

Other Reagents Required

Human CD47 DuoSet ELISA Scientific Data Examples

Preparation and Storage

Background: CD47

Citations (1)

Customer Reviews (1)

Human CD47 DuoSet ELISA

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

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