Human BMP-6 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY507
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
156-10000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human BMP-6 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human BMP-6 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human BMP-6. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human BMP-6 DuoSet ELISA
Human BMP-6 ELISA Standard Curve
Kit Contents for Human BMP-6 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
Normal Goat Serum: (Catalog # DY005)
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Normal Goat Serum: (Catalog # DY005)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BMP-6
BMP-6 (Bone Morphogenetic Protein 6) forms disulfide-linked homodimers and heterodimers with BMP-2 that signal through complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) receptors. BMP-6 promotes osteoblast differentiation from mesenchymal stem cells, chondrocyte maturation, Ang II-induced aldosterone production in the adrenal cortex, and hormone production and responsiveness in ovarian granulosa cells. It promotes neurite outgrowth, neuroprotection, angiogenesis, immune tolerance, and tumor progression. BMP-6 binds to RGM-C/Hemojuvelin to enhance Hepcidin expression and prevent serum iron overload. Heterodimers of BMP-2 and BMP-6 show increased potency at inducing bone formation compared to either BMP-2 or BMP-6 homodimers.
Long Name
Bone Morphogenetic Protein 6
Alternate Names
BMP6, Vgr-1
Gene Symbol
BMP6
Additional BMP-6 Products
Product Documents for Human BMP-6 DuoSet ELISA
Product Specific Notices for Human BMP-6 DuoSet ELISA
For research use only
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