Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA New
R&D Systems, part of Bio-Techne | Catalog # QK0124
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Cell Lysates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
5.23 pg/mL
Assay Range
18.8-37.5 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
The Quantikine QuicKit Human BAFF/BlyS/TNFSF13B Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human BAFF levels in cell culture supernates, serum, plasma, and cell lysates. It contains recombinant human BAFF and antibodies raised against the recombinant protein. Results obtained for naturally occurring human BAFF showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human BAFF.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human BAFF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 81.2 | 605 | 96.2 | 558 |
| Standard Deviation | 2.28 | 21.9 | 10.5 | 34.3 |
| CV% | 2.8 | 3.6 | 10.9 | 6.2 |
Recovery for Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
The recovery of BAFF is spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 97 | 85-105 |
| EDTA Plasma (n=2) | 101 | 85-110 |
| Heparin Plasma (n=2) | 99 | 81-108 |
| Lysis Buffer (n=1) | 125 | 117-133 |
| Serum (n=2) | 95 | 78-104 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of BAFF were diluted with Calibrator Diluent RD5-20 (diluted 1:2) to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BAFF/BLyS/TNFSF13B
BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.
Long Name
B cell Activating Factor
Alternate Names
BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4
Gene Symbol
TNFSF13B
Additional BAFF/BLyS/TNFSF13B Products
Product Documents for Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
Product Specific Notices for Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
For research use only
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