Human Angiopoietin-like 4 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY3485
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
1.25-80 ng/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Angiopoietin-like 4 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Angiopoietin-like 4 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiopoietin-like4. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Angiopoietin-like 4 DuoSet ELISA
Human Angiopoietin-like Protein 4 / ANGPTL4 ELISA Standard Curve
Human Angiopoietin-like 4 DuoSet ELISA
Levels of ANGPTL4 in sera from RA patients and controls after correction for the false positive reactions to rheumatoid factor (RF).(A) ANGPTL4 was measured in sera from 57 patients with rheumatoid arthritis (RA) and 43 matched controls by ELISA using DY3485 kit with lot IV detection antibody. (B) ANGPTL4 was measured as in (A) in sera from RF+ (n = 33) and RF- (n = 20) patients with RA. The means ± SD are indicated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28107351), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Angiopoietin-like Protein 4/ANGPTL4 by ELISA
Levels of ANGPTL4 in sera from RA patients and controls after correction for the false positive reactions to rheumatoid factor (RF).(A) ANGPTL4 was measured in sera from 57 patients with rheumatoid arthritis (RA) and 43 matched controls by ELISA using DY3485 kit with lot IV detection antibody. (B) ANGPTL4 was measured as in (A) in sera from RF+ (n = 33) and RF- (n = 20) patients with RA. The means ± SD are indicated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28107351), licensed under a CC-BY license. Not internally tested by R&D Systems.Kit Contents for Human Angiopoietin-like 4 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiopoietin-like Protein 4/ANGPTL4
Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated. The coiled coil domain mediates the formation of variable sized disulfide-linked oligomers. This domain directly inhibits lipoprotein lipase (LPL), resulting in increased circulating triglyceride levels. ANGPTL4 circulates in association with HDL lipoproteins. Its expression in adipose tissue is induced by fasting and suppressed by feeding. In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells. The N-terminal fragment can function as an angiogenesis inhibitor. In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins alpha 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing. ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells.
Alternate Names
Angiopoietin like Protein 4, ANGPTL4, FIAF, HFARP, PGAR
Gene Symbol
ANGPTL4
Additional Angiopoietin-like Protein 4/ANGPTL4 Products
- All Products for Angiopoietin-like Protein 4/ANGPTL4
- Angiopoietin-like Protein 4/ANGPTL4 cDNA Clones
- Angiopoietin-like Protein 4/ANGPTL4 ELISA Kits
- Angiopoietin-like Protein 4/ANGPTL4 Lysates
- Angiopoietin-like Protein 4/ANGPTL4 Primary Antibodies
- Angiopoietin-like Protein 4/ANGPTL4 Proteins and Enzymes
- Angiopoietin-like Protein 4/ANGPTL4 Simple Plex
Product Documents for Human Angiopoietin-like 4 DuoSet ELISA
Product Specific Notices for Human Angiopoietin-like 4 DuoSet ELISA
For research use only
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