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StemXVivo Serum-Free MSC Freezing Media

R&D Systems, Inc. a Bio-Techne Brand

For serum-free cryopreservation of Human/Mouse/Rat Mesenchymal Stem Cells (MSCs)

Key Product Details

MSC Viability Following Cryopreservation in StemXVivo®Serum-Free MSC Freezing Media.
(2)
MSC Identification Following Cryopreservation in StemXVivo® Serum-Free MSC Freezing Media. 
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CCM016
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, MSCs can be cryopreserved for long-term storage using the following protocol:

  • MSCs are detached from the culture dish
  • MSCs are resuspended in StemXVivo® Serum-Free MSC Freezing Media
  • Frozen, cryopreserved MSCs are stored in liquid nitrogen
 

 

Reagents Provided

Reagents supplied in the Human StemXVivo® Serum-Free Mesenchymal Stem Cell Freezing Media (Catalog # CCM016):

  • 50 mL of serum-free MSC freezing media

 

Other Supplies Required

Reagents

  • Human StemXVivo® Serum-Free MSC Expansion Media (Catalog # CCM014 or equivalent)
  • TrypLE Express (Invitrogen® or equivalent)

Materials

  • Human MSCs
  • Tissue culture flasks
  • 15 mL centrifuge tubes
  • Cryogenic storage vials
  • Pipettes and pipette tips
  • Serological pipettes

Equipment

  • 37 °C and 5% CO2 incubator
  • -80 °C freezer
  • Liquid nitrogen storage
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 37 °C water bath

 

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be modified.

Freezing

Detach the MSCs from the culture dish using a dissociation solution.

Detach the MSCs from the culture dish using a dissociation solution

Transfer the dissociated cells to a 15 mL conical tube.

Centrifuge the MSCs at 200 x g for 5 minutes.

Transfer the dissociated cells to a 15 mL conical tube

Resuspend the cell pellet in cold StemXVivo® Serum-Free MSC Freezing Media.

Transfer the cells to a cryovial

Transfer the MSC suspension to a cryovial immediately.

Place the cryovial in a cryogenic container at -80 °C overnight.

Store the frozen cryovial in liquid nitrogen.

Transfer the MSC suspension to a cryovial immediately
 

Thawing of Cryopreserved Stem Cells

Remove the frozen vials from the liquid nitrogen, and rapidly thaw in a 37 °C water bath.

Remove the frozen vials from the liquid nitrogen, and rapidly thaw in a 37C water bath

Transfer the cells into a 15 mL centrifuge tube containing pre-warmed StemXVivo® Mesenchymal Stem Cell Expansion Media.

Centrifuge the MSCs at 200 x g for 5 minutes.

Transfer the cells into a 15 mL centrifuge tube containing pre-warmed StemXVivo Mesenchymal Stem Cell Expansion Media

Resuspend the cells in serum-free complete growth medium and plate the MSCs in desired culture conditions.

Resuspendthe cells in serum-free complete growth medium and plate the MSCs in desired culture conditions

Citations for StemXVivo Serum-Free MSC Freezing Media


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FAQs for StemXVivo Serum-Free MSC Freezing Media

  • Can Mesenchymal Stem Cells (MSCs) grown in StemXVivo® MSC Expansion Media (Catalog # CCM004) be cryopreserved?

    MSCs grown in StemXVivo® MSC Expansion Media can be cryopreserved using appropriate cryopreservation media, such as StemXVivo® Serum-Free MSC Freezing Media (Catalog # CCM016) or equivalent.

Product Documents for StemXVivo Serum-Free MSC Freezing Media

Certificate of Analysis

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