Cultrex Synthetic Hydrogel Kit (RUO), 1 mL

Differentiation of intestinal organoids in Cultrex Synthetic Hydrogel. Organoids expanded in Cultrex Synthetic Hydrogel exhibit expected cystic morphology, indicative of healthy baseline development. Upon differentiation, the organoids begin to lose their cystic morphology and start forming more specialized, structured tissues. This demonstrates the ability of Cultrex Synthetic Hydrogel to support both healthy initial organoid growth and subsequent differentiation into mature structures.

Cultrex Synthetic Hydrogel performance compared to basement membrane extracts. Cultrex UltiMatrix BME (Catalog # BME001-05) domes were plated at 10,000 cell per 10 μL hydrogel, and Cultrex Synthetic Hydrogel (Catalog # CSH-RUO) domes were plated at 15,000 cells. After three days in culture, comparable organoid growth is observed.

Cultrex Synthetic Hydrogel performance compared to competitor basement membrane extracts. Domes using animal component derived matrix from another supplier and Cultrex Synthetic Hydrogel (Catalog # CSH-RUO) were each plated at 10,000 cell per 10 μL hydrogel. Morphologically similar organoid growth is observed after 4 and 5.5 days, respectively.

Cultrex Synthetic Hydrogel outperforms alternative synthetic matrices. Successful organoid growth observed in Cultrex Synthetic Hydrogel (Catalog # CSH-RUO-01) while organoids encapsulated in a competitor's defined hydrogel showed failed organoid formation and significant cell death by day 4.