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Proteome Profiler Rat Cytokine Array Kit, Panel A
R&D Systems, Inc. a Bio-Techne Brand
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The Rat Cytokine Array detects multiple analytes in cell culture supernates. NR8383 rat alveolar macrophage cells were either untreated or treated as below. Data shown are from a two minute exposure to X-ray film. A, untreated. B, Treated with 100 ng/mL LPS for 24 hours. C, Pre-treated with 100 nM Dexamethasone for 72 hours followed by treatment with 100 ng/mL LPS for 24 hours. |
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The Rat Cytokine Array detects multiple analytes in tissue lysates and serum. A rat was injected with 0.1 mg/kg LPS. After 24 hours, tissues were excised and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film (Panels A-B). Tissues were excised from untreated rats and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film (Panels C-E). Serum samples from 15 week old male rats were prepared as described in the Sample Collection and Storage section. 100 µL of serum was run on the array. Data shown are from a 5 minute exposure to X-ray film (Panel F). |
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The Rat Cytokine Array detects multiple analytes in cell culture supernates and lysates. (A. and B.) Supernates from NR8383 rat alveolar macrophage cell line untreated or treated with 50 ng/mL LPS for 24 hours. 500 μL of supernate was used for each array shown. (C. and D.) Lysates from NR8383 rat alveolar macrophage cell line untreated or treated with 50 ng/mL LPS for 24 hours. 200 μg of lysates was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System. |
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The Rat Cytokine Array detects cytokines present in serum and tissue. Representative membranes and selective quantification are shown for rat serum (A., 150 μL), lysates of rat lung tissue (B., 400 μg), and lysates of rat liver tissue (C., 400 μg). Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System. |
Citations for Proteome Profiler Rat Cytokine Array Kit, Panel A (43)
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Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Rat Cytokine Array Kit, Panel A include:
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Species: Rat
Sample Types: Tissue Homogenates
B Zhang et al. (2021), Peripheral Macrophage-derived Exosomes promote repair after Spinal Cord Injury by inducing Local Anti-inflammatory type Microglial Polarization via Increasing Autophagy International journal of biological sciences, 17(5):1339-1352.
PMID: 33867850 -
Species: Rat
Sample Types: Tissue Homogenates
DK Hong et al. (2021), Transient Global Ischemia-Induced Brain Inflammatory Cascades Attenuated by Targeted Temperature Management International Journal of Molecular Sciences, 22(10).
PMID: 34066051 -
Species: Rat
Sample Types: Cell Culture Supernates
F Yergöz et al. (2021), Adenine Nucleotide Translocase 1 Expression Modulates the Immune Response in Ischemic Hearts Cells, 10(8).
PMID: 34440901 -
Species: Rat
Sample Types: Cell Lysates
L Yang et al. (2021), Effects of prenatal photobiomodulation treatment on neonatal hypoxic ischemia in rat offspring Theranostics, 11(3):1269-1294.
PMID: 33391534 -
Species: Rat
Sample Types: Cell Culture Supernates
EGF Filho et al. (2021), RACK1 plays a critical role in mast cell secretion and Ca2+ mobilization by modulating F-actin dynamics Journal of Cell Science, 134(9).
PMID: 34550354 -
Species: Human
Sample Types: Tissue Homogenates
C Engelmann et al. (2020), Recombinant Alkaline Phosphatase Prevents Acute on Chronic Liver Failure Sci Rep, 10(1):389.
PMID: 31942020 -
Species: Rat
Sample Types: Cell Culture Supernates
C Zogbi et al. (2020), Beneficial effects of IL-4 and IL-6 on rat neonatal target cardiac cells Sci Rep, 10(1):12350.
PMID: 32704142 -
Species: Rat
Sample Types: Cell Culture Supernates
F Rohn et al. (2020), Impaired integrin &alpha5 /&beta1 -mediated hepatocyte growth factor release by stellate cells of the aged liver Aging Cell, 0(0):e13131.
PMID: 32157808 -
Species: Rat
Sample Types: Cell Lysates
W Mu et al. (2020), Tspan8-Tumor Extracellular Vesicle-Induced Endothelial Cell and Fibroblast Remodeling Relies on the Target Cell-Selective Response Cells, 9(2).
PMID: 32013145 -
Species: Rat
Sample Types: Protein
X Zong et al. (2020), Theta-burst transcranial magnetic stimulation promotes stroke recovery by vascular protection and neovascularization Theranostics, 10(26):12090-12110.
PMID: 33204331 -
Species: Rat
Sample Types: Cell Culture Supernates
S Afroz et al. (2019), CGRP Induces Differential Regulation of Cytokines from Satellite Glial Cells in Trigeminal Ganglia and Orofacial Nociception Int J Mol Sci, 20(3).
PMID: 30736422 -
Species: Rat
Sample Types: Cell Culture Supernates
SZ Xie et al. (2019), Dendrobium huoshanense polysaccharide regulates intestinal lamina propria immune response by stimulation of intestinal epithelial cells via toll-like receptor 4 Carbohydr Polym, 222(0):115028.
PMID: 31320099 -
Species: Rat
Sample Types: Tissue Homogenates
JCT Chio et al. (2019), The effects of human immunoglobulin G on enhancing tissue protection and neurobehavioral recovery after traumatic cervical spinal cord injury are mediated through the neurovascular unit J Neuroinflammation, 16(1):141.
PMID: 31288834 -
Species: Rat
Sample Types: Tissue Homogenates
P Basu et al. (2019), Euphorbia bicolor (Euphorbiaceae) Latex Extract Reduces Inflammatory Cytokines and Oxidative Stress in a Rat Model of Orofacial Pain Oxid Med Cell Longev, 2019(0):8594375.
PMID: 31612077 -
Species: Rat
Sample Types: Tissue Homogenates
YM Song et al. (2019), Differences in pathological changes between two rat models of severe traumatic brain injury Neural Regen Res, 14(10):1796-1804.
PMID: 31169198 -
Species: Rat
Sample Types: Cell Culture Supernates
D Cizkova et al. (2018), Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Medium Improves Functional Recovery in a Rat Model of Spinal Cord Injury Int J Mol Sci, 19(3).
PMID: 29543759 -
Species: Rat
Sample Types: Cell Culture Supernates
SA O'Sullivan et al. (2018), Sphingosine 1-phosphate receptors regulate TLR4-induced CXCL5 release from astrocytes and microglia J. Neurochem., 0(0).
PMID: 29377126 -
Species: Rat
Sample Types: Cell Culture Supernates
M Duhamel et al. (2018), Paclitaxel�treatment and PC1/3 knockdown in macrophages is a promising anti-glioma strategy as revealed by proteomics and cytotoxicity studies Mol. Cell Proteomics, 0(0).
PMID: 29531019 -
Species: Rat
Sample Types: Cell Culture Supernates
A Rutkowska et al. (2018), EBI2 regulates pro-inflammatory signalling and cytokine release in astrocytes Neuropharmacology, 133(0):121-128.
PMID: 29374507 -
Species: Rat
B Corradetti et al. (2017), Immune tuning scaffold for the local induction of a pro-regenerative environment Sci Rep, 7(1):17030.
PMID: 29208986 -
Species: Rat
Sample Types: Serum
MP Saldías et al. (2017), Aged blood factors decrease cellular responses associated with delayed gingival wound repair PLoS ONE, 12(9):e0184189.
PMID: 28898261 -
Species: Rat
Sample Types: Cell Culture Supernates
SA Liddelow et al. (2017), Neurotoxic reactive astrocytes are induced by activated microglia Nature, 541(7638):481-487.
PMID: 28099414 -
Species: Rat
Sample Types: Cell Lysates
E Elgström et al. (2017), Cytokine evaluation in untreated and radioimmunotherapy-treated tumors in an immunocompetent rat model Tumour Biol., 39(4):1010428317697.
PMID: 28381167 -
Species: Rat
Sample Types: Cell Culture Supernates
EZ da Silva et al. (2017), Adaptor protein-3: A key player in RBL-2H3 mast cell mediator release PLoS ONE, 12(3):e0173462.
PMID: 28273137 -
Species: Rat
Sample Types: Plasma
J Lagrange et al. (2017), Implication of Free Fatty Acids in Thrombin Generation and Fibrinolysis in Vascular Inflammation in Zucker Rats and Evolution with Aging Front Physiol, 8(0):949.
PMID: 29213245 -
Species: Rat
Sample Types: Tissue Homogenates
N Gupta et al. (2017), Activation of NLRP3 inflammasome complex potentiates venous thrombosis in response to hypoxia Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 28420787 -
Species: Rat
Sample Types: Serum
T Maerz et al. (2017), Acute Mobilization and Migration of Bone Marrow-derived Stem Cells following Anterior Cruciate Ligament Rupture Osteoarthr. Cartil, 0(0).
PMID: 28284998 -
Species: Rat
Sample Types: Cell Culture Supernates
Gerald Ponath (2016), Myelin phagocytosis by astrocytes after myelin damage promotes lesion pathology Brain, 0(0).
PMID: 28007993 -
Species: Rat
Sample Types: Tissue Homogenates
Chandan K Sen (2016), Robot-assisted mechanical therapy attenuates stroke-induced limb skeletal muscle injury FASEB J, 0(0).
PMID: 27895105 -
Species: Rat
Sample Types: Tissue Homogenates
Paul L Durham (2016), Tumor necrosis factor-Alpha stimulates cytokine expression and transient sensitization of trigeminal nociceptive neurons Arch. Oral Biol., 0(0).
PMID: 27836101 -
Species: Rat
Sample Types: Tissue Homogenates
Takuro Shirasu (2016), Nanoparticles Effectively Target Rapamycin Delivery to Sites of Experimental Aortic Aneurysm in Rats PLoS ONE, 11(6):e0157813.
PMID: 27336852 -
Species: Mouse
Sample Types: Tissue Homogenates
(2016), Ocular Delivery of PACAP1-27 Protects the Retina From Ischemic Damage in Rodents Invest. Ophthalmol. Vis. Sci, 57(15):6683-6691.
PMID: 27936470 -
Species: Rat
Sample Types: Cell Lysates
Andy T Y Lau (2016), Aberrant cytokine secretion and zinc uptake in chronic cadmium-exposed lung epithelial cells Proteomics Clin Appl, 0(0).
PMID: 27801555 -
Species: Rat
Sample Types: Cell Culture Supernates
Deliyanti D et al. (2015), FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy. PLoS ONE, 10(7):e0134392.
PMID: 26222724 -
Species: Rat
Sample Types: Serum
Altara et al. (2014), Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample. BMC Biotechnol, 14(0):63.
PMID: 25022797 -
Species: Rat
Sample Types: Tissue Homogenates
Kiss T et al. (2014), Novel mechanisms of sildenafil in pulmonary hypertension involving cytokines/chemokines, MAP kinases and Akt. PLoS ONE, 9(8):e104890.
PMID: 25133539 -
Species: Rat
Sample Types: Cell Culture Supernates
Klinkhammer B et al. (2014), Mesenchymal stem cells from rats with chronic kidney disease exhibit premature senescence and loss of regenerative potential. PLoS ONE, 9(3):e92115.
PMID: 24667162 -
Species: Rat
Sample Types: Tissue Homogenates
Tolg C et al. (2012), A RHAMM mimetic peptide blocks hyaluronan signaling and reduces inflammation and fibrogenesis in excisional skin wounds. Am J Pathol, 181(4):1250-70.
PMID: 22889846 -
Species: Rat
Sample Types: Tissue Homogenates
van Koppen A et al. (2012), Human embryonic mesenchymal stem cell-derived conditioned medium rescues kidney function in rats with established chronic kidney disease. PLoS ONE, 7(6):e38746.
PMID: 22723882 -
Species: Rat
Sample Types: Cell Culture Supernates
Gibbings S et al. (2011), Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, peroxisome proliferator-activated receptor-gamma sumoylation, and HIF-1alpha. J. Biol. Chem., 286(2):961-75.
PMID: 21059659 -
Species: Rat
Sample Types: Serum
Hegde NV et al. (2011), A postweaning iron-adequate diet following neonatal iron deficiency affects iron homeostasis and growth in young rats. J. Nutr., 141(3):386-90.
PMID: 21209256 -
Species: Rat
Sample Types: Cell Culture Supernates
Lu Y et al. (2011), Treatment of experimental adjuvant arthritis with a novel folate receptor-targeted folic acid-aminopterin conjugate. Arthritis Res. Ther., 13(2):R56.
PMID: 21463515 -
Species: Rat
Sample Types: Cell Culture Supernates
Song X et al. (2009), Construction and characterization of a novel DNA vaccine that is potent antigen-specific tolerizing therapy for experimental arthritis by increasing CD4+CD25+Treg cells and inducing Th1 to Th2 shift in both cells and cytokines. Vaccine, 27(5):690-700.
PMID: 19095031
There are no citations that match your criteria.
Customer Reviews for Proteome Profiler Rat Cytokine Array Kit, Panel A (8)
4.5 out of 5
8 customer ratings
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Verified Customer | Posted 03/11/2021
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Verified Customer | Posted 11/15/2020
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Verified Customer | Posted 05/15/2020This kit is easy to use and would definitely use again.
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Verified Customer | Posted 02/01/2020Performed well, great tool to screen for possible targets before making a more quantitative and more expensive assay. Spots can be easily evaluated using e.g., Bio-Rad ImageLab, and the semi-quantitative analysis can basically detect substantial differences between experimental groups.
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Verified Customer | Posted 04/01/2018
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Verified Customer | Posted 01/20/2017This array kit is a very nice tool to screen potential cytokines in spent media of cell culture.
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Verified Customer | Posted 01/03/2017This kit is an excellent tool in screening potential cytokines and chemokines. However, it would be nice if more targets can be included for this Rat cytokine panel (since the mouse and human panel has XL format).
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Verified Customer | Posted 10/06/2016
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FAQs for Proteome Profiler Rat Cytokine Array Kit, Panel A
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
