Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

Name: Proteome Profiler Rat Cytokine Array Kit, Panel A
Brand: R&D Systems
SKU: ARY008
Rating: 5 (8 reviews)

The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.

Proteome Profiler Rat Cytokine Array Kit, Panel A Best Seller

R&D Systems, part of Bio-Techne | Catalog # ARY008

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Product Datasheet / COA / SDS

Contains 4 membranes - each spotted in duplicate with 29 different cytokine antibodies

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Product Summary
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Rat

Product Summary for Proteome Profiler Rat Cytokine Array Kit, Panel A

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected rat cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.

Troubleshooting Guide

Key Benefits

Detects 29 rat cytokines and chemokines simultaneously
Requires no specialized equipment
Compatible with LI-COR* and chemiluminescence detection

*If using a LI-COR, additional reagents and protocol modifications are required. Refer to protocol.

Principle of the Assay

The Proteome Profiler Rat Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

Kit Contents

Rectangular 4-Well Multi-dish
4 Rat Cytokine Array, Panel A nitrocellulose membranes spotted with 29 different antibodies to rat cytokines and chemokines
Array Buffer 4
Array Buffer 6
Chemi Reagent 1
Chemi Reagent 2
Detection Antibody Cocktail, Rat Cytokine Array, Panel A
Streptavidin-HRP
Transparency Overlay Template
Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 ^°C to 8 ^°C. Do not use past kit expiration date.

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
CXCL1/CINC-1	IL-1ra/IL-1F3	L-Selectin
CXCL3/CINC-2 alpha/beta	IL-2	CXCL9/MIG
CXCL2/CINC-3	IL-3	CCL3/MIP-1 alpha
CNTF	IL-4	CCL20/MIP-3 alpha
Fractalkine/CXC3CL1	IIL-6	CCL5/RANTES
GM-CSF	IL-10	CXCL7/Thymus Chemokine
ICAM-1	IL-13	TIMP-1
IFN-gamma	IL-17	TNF-alpha
IL-1 alpha/IL-1F1	CXCL10/IP-10	VEGF
IL-1 beta/IL-1F2	LIX

Assays for Analytes represented in the Rat Cytokine Array Kit, Panel A

Analyte	Quantikine^® ELISA Kits	DuoSet^® ELISA Development Systems
CXCL1/CINC-1	RCN100	DY515
CXCL3/CINC-2 alpha/beta	RCN200	DY516
CXCL2/CINC-3	RCN300	DY525
CNTF		DY557
Fractalkine/CX3CL1	MCX310	DY537
GM-CSF		DY518
ICAM-1	RIC100	DY583
IFN-gamma	RIF00	DY585
IL-1 alpha	RRA00	DY500
IL-1 beta	RLB00	DY501
IL-1ra
IL-2	R2000	DY502
IL-3
IL-4	R4000	DY504
IL-6	R6000B	DY506
IL-10	R1000	DY522
IL-13
IL-17
CXCL10/IP-10
LIX		DY543
L-Selectin		DY1534
CXCL9/MIG
CCL3/MIP-1 alpha
CCL20/MIP-3 alpha		DY540
CCL5/RANTES	MMR00
CXCL7/Thymus Chemokine		DY1116
TIMP-1	RTM100	DY580
TNF-alpha	RTA00	DY510
VEGF	RRV00	DY564

Scientific Data Images for Proteome Profiler Rat Cytokine Array Kit, Panel A

The Rat Cytokine Array detects multiple analytes in cell culture supernates.

NR8383 rat alveolar macrophage cells were either untreated or treated as below. Data shown are from a two minute exposure to X-ray film. A, untreated. B, Treated with 100 ng/mL LPS for 24 hours. C, Pre-treated with 100 nM Dexamethasone for 72 hours followed by treatment with 100 ng/mL LPS for 24 hours.

The Rat Cytokine Array detects multiple analytes in tissue lysates and serum.

A rat was injected with 0.1 mg/kg LPS. After 24 hours, tissues were excised and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film (Panels A-B). Tissues were excised from untreated rats and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film (Panels C-E). Serum samples from 15 week old male rats were prepared as described in the Sample Collection and Storage section. 100 µL of serum was run on the array. Data shown are from a 5 minute exposure to X-ray film (Panel F).

The Rat Cytokine Array detects multiple analytes in cell culture supernates and lysates.

(A. and B.) Supernates from NR8383 rat alveolar macrophage cell line untreated or treated with 50 ng/mL LPS for 24 hours. 500 μL of supernate was used for each array shown. (C. and D.) Lysates from NR8383 rat alveolar macrophage cell line untreated or treated with 50 ng/mL LPS for 24 hours. 200 μg of lysates was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

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Formulation, Preparation, and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Multiplex Cytokine Assay Kits

Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex^® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.