Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # RRA00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL)
Sensitivity
4.12 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Product Summary for Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit
The Quantikine Rat IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-1 alpha accurately. Results obtained using natural rat IL-1 alpha showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-1 alpha.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Rat
Specificity
Natural and recombinant rat IL-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 54 | 134 | 417 | 54 | 130 | 449 |
| Standard Deviation | 3.6 | 6.3 | 16.5 | 5.0 | 9.5 | 29.7 |
| CV% | 6.7 | 4.7 | 4.0 | 9.3 | 7.3 | 6.6 |
Recovery for Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit
The recovery of rat IL-1 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 104 | 100-111 |
| EDTA Plasma (n=5) | 98 | 89-112 |
| Serum (n=4) | 92 | 85-99 |
Linearity
To assess the linearity of the assay, samples spiked with rat IL-1 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit
Rat IL-1 alpha/IL-1F1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 alpha/IL-1F1
IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signalling.
Long Name
Interleukin 1 alpha
Alternate Names
BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM
Entrez Gene IDs
Gene Symbol
IL1A
Additional IL-1 alpha/IL-1F1 Products
Product Documents for Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit
Product Specific Notices for Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.