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Proteome Profiler Human Phospho-Immunoreceptor Array Kit
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
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The Human Phospho-Immunoreceptor Array detects multiple tyrosine phosphorylated immunoreceptors in lysates prepared from cells activated by antibody mediated cross-linking of cell-surface immunoreceptors. Jurkat human acute T cell leukemia cells were incubated with a mouse monoclonal anti-CD3 epsilon antibody (R&D Systems, Catalog # MAB100) or mouse IgG1 isotype control (R&D Systems, Catalog # MAB002) followed by incubation with a goat anti-mouse antibody (R&D Systems, Catalog # AF007) for 5 minutes. 100 µg of lysate was run on each array. |
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The Human Phospho-Immunoreceptor Array detects multiple tyrosine phosphorylated immunoreceptors in lysates prepared from cells activated by antibody mediated cross-linking of cell-surface immunoreceptors. THP-1 human acute monocytic leukemia cells were incubated with goat anti-Fc gamma RIIA antibody (R&D Systems, Catalog # AF1875) or normal goat IgG (R&D Systems, Catalog # AB-108-C) followed by incubation with a donkey anti-goat IgG antibody (R&D Systems, Catalog # AF109) for 5 minutes [Maresco, D.L. et al. (1999) J. Immunol. 162:6458]. |
Citations for Proteome Profiler Human Phospho-Immunoreceptor Array Kit (7)
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Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Human Phospho-Immunoreceptor Array Kit include:
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Species: Human
Sample Types: Cell Lysates
T Sinthuwiwa et al. (2022), A LILRB1 variant with a decreased ability to phosphorylate SHP-1 leads to autoimmune diseases Scientific Reports, 12(1):15420.
PMID: 36104364 -
Species: Human
Sample Types: Cell Lysates
S Herbster et al. (2021), Low RECK Expression Is Part of the Cervical Carcinogenesis Mechanisms Cancers, 13(9).
PMID: 34066355 -
Species: Human
Sample Types: Cell Lysates
S Durlanik et al. (2021), CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1 Scientific Reports, 11(1):14849.
PMID: 34290311 -
Species: Human
Sample Types: Cell Lysates
AO Achieng et al. (2019), Molecular basis of reduced LAIR1 expression in childhood severe malarial anaemia: Implications for leukocyte inhibitory signalling EBioMedicine, 45(0):278-289.
PMID: 31257148 -
Species: Human
Sample Types: Cell Lysates
M Son et al. (2017), Evidence for C1q-mediated crosslinking of CD33/LAIR-1 inhibitory immunoreceptors and biological control of CD33/LAIR-1 expression Sci Rep, 7(1):270.
PMID: 28325905 -
Species: Human
Sample Types: Cell Lysates
Kotani N et al. (2012), Fibroblast Growth Factor Receptor 3 (FGFR3) Associated with the CD20 Antigen Regulates the Rituximab-induced Proliferation Inhibition in B-cell Lymphoma Cells. J Biol Chem, 287(44):37109-18.
PMID: 22932894 -
Species: Human
Sample Types: Cell Lysates
Atanackovic D et al. (2011), Surface molecule CD229 as a novel target for the diagnosis and treatment of multiple myeloma. Haematologica, 0(0).
PMID: 21606160
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Customer Reviews for Proteome Profiler Human Phospho-Immunoreceptor Array Kit (1)
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Verified Customer | Posted 09/23/2021very good product, easy protocol, good results. it can be kept in 4-degree fridge.
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FAQs for Proteome Profiler Human Phospho-Immunoreceptor Array Kit
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The insert states to lyse 1 x 107 cells in 1 mL Lysis Buffer 17. What is the approximate protein concentration of the resulting lysate?
Lysing following recommendations in the product insert produces a lysate with a protein concentration of about 1.5-2.0 mg/mL. Quantitation of sample protein concentration using a total protein assay is recommended.
